Decreased expression of Src homology 2 domain-containing protein tyrosine phosphatase 1 reduces T cell activation threshold but not the severity of experimental autoimmune myasthenia gravis

Caishu Deng, Bo Wu, Huan Yang, Rehana Z. Hussain, Amy E. Lovett-Racke, Premkumar Christadoss, Michael K. Racke

Research output: Contribution to journalArticle

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Abstract

Myasthenia gravis (MG) and its murine model experimental autoimmune myasthenia gravis (EAMG) are T cell-dependent, antibody-mediated autoimmune diseases. Src homology 2 domain-containing protein tyrosine phosphatase 1 (SHP-1) is a cytosolic tyrosine phosphatase that is involved in regulating the T cell activation cascade from signals initiated through the TCR. To study the role of SHP-1 in EAMG pathogenesis, we immunized C57BL/6 (B6) mice heterozygous for deletion of the SHP-1 gene (mev+/-) and their littermate wild type B6 mice with torpedo acetylcholine receptor (TAChR). T cell proliferation and IFNγ production were significantly increased in B6.mev+/- mice after immunization with AChR compared to that of wild type littermates. However, clinical incidence and severity of the disease were not changed. There also were no significant differences in AChR-specific antibodies produced between wild type and mev+/- mice. These data suggest that deficiency in SHP-1 expression does decrease the activation threshold of autoreactive T cells in EAMG, but the increased frequency of autoreactive T cells does not aggravate EAMG in terms of clinical score, incidence, or antibody titers.

Original languageEnglish
Pages (from-to)76-82
Number of pages7
JournalJournal of Neuroimmunology
Volume138
Issue number1-2
DOIs
StatePublished - May 2003

Fingerprint

SH2 Domain-Containing Protein Tyrosine Phosphatases
Autoimmune Experimental Myasthenia Gravis
Protein Phosphatase 1
Protein Tyrosine Phosphatases
T-Lymphocytes
Antibodies
Torpedo
Myasthenia Gravis
Incidence
Cholinergic Receptors
Phosphoric Monoester Hydrolases
Autoimmune Diseases
Tyrosine
Immunization
Cell Proliferation
Genes

Keywords

  • Acetylcholine receptor
  • Auto-antibody
  • Experimental autoimmune myasthenia gravis
  • T cell activation
  • Tyrosine phosphatase

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Decreased expression of Src homology 2 domain-containing protein tyrosine phosphatase 1 reduces T cell activation threshold but not the severity of experimental autoimmune myasthenia gravis. / Deng, Caishu; Wu, Bo; Yang, Huan; Hussain, Rehana Z.; Lovett-Racke, Amy E.; Christadoss, Premkumar; Racke, Michael K.

In: Journal of Neuroimmunology, Vol. 138, No. 1-2, 05.2003, p. 76-82.

Research output: Contribution to journalArticle

Deng, Caishu ; Wu, Bo ; Yang, Huan ; Hussain, Rehana Z. ; Lovett-Racke, Amy E. ; Christadoss, Premkumar ; Racke, Michael K. / Decreased expression of Src homology 2 domain-containing protein tyrosine phosphatase 1 reduces T cell activation threshold but not the severity of experimental autoimmune myasthenia gravis. In: Journal of Neuroimmunology. 2003 ; Vol. 138, No. 1-2. pp. 76-82.
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