Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis

Luca Cicalese, Richard H. Duerr, Michael A. Nalesnik, Peter F. Heeckt, Kenneth K W Lee, Wolfgang H. Schraut

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Patients with chronic ulcerative colitis (CUC) are known to have decreased spontaneous IgA secretion by colonic mononuclear cells. The aim of this study was to determine whether a similar alteration exists in the apparently healthy ileum of patients with CUC. The concentration of IgA was measured in the supernatant from homogenized mucosal ileal biopsies using a sandwich-type ELISA. The concentration of IgA was significantly (P=0.025) decreased in the ileum of patients with CUC (N=24) in comparison to normal ileum (N=10). The number of mucosal IgA-containing mononuclear cells (MNC) was also determined using an avidin-biotin-immunoperoxidase technique on paraffin-embedded ileal sections. Although reduced, the number of positive cells and their distribution was not significantly different in the ileum of patients with CUC (N=20) when compared to normal ileum (N=10). We suggest that decreased mucosal IgA levels are a panintestinal condition in CUC and that this is a primary alteration rather than a secondary response to the inflammatory process. Considering the role of IgA, we propose that decreased mucosal IgA levels in CUC may predispose to the disease by a reduction of the immune-mediated exclusion mechanism and/or by an impairment of the down-regulation of the inflammatory response.

Original languageEnglish (US)
Pages (from-to)805-811
Number of pages7
JournalDigestive Diseases and Sciences
Volume40
Issue number4
DOIs
StatePublished - Apr 1995
Externally publishedYes

Fingerprint

Ulcerative Colitis
Ileum
Immunoglobulin A
Avidin
Biotin
Immunoenzyme Techniques
Paraffin
Down-Regulation
Cell Count
Enzyme-Linked Immunosorbent Assay
Biopsy

Keywords

  • ileum
  • immunoglobulin A
  • ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Cicalese, L., Duerr, R. H., Nalesnik, M. A., Heeckt, P. F., Lee, K. K. W., & Schraut, W. H. (1995). Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis. Digestive Diseases and Sciences, 40(4), 805-811. https://doi.org/10.1007/BF02064983

Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis. / Cicalese, Luca; Duerr, Richard H.; Nalesnik, Michael A.; Heeckt, Peter F.; Lee, Kenneth K W; Schraut, Wolfgang H.

In: Digestive Diseases and Sciences, Vol. 40, No. 4, 04.1995, p. 805-811.

Research output: Contribution to journalArticle

Cicalese, Luca ; Duerr, Richard H. ; Nalesnik, Michael A. ; Heeckt, Peter F. ; Lee, Kenneth K W ; Schraut, Wolfgang H. / Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis. In: Digestive Diseases and Sciences. 1995 ; Vol. 40, No. 4. pp. 805-811.
@article{a350ef390014498dac46258137e0f459,
title = "Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis",
abstract = "Patients with chronic ulcerative colitis (CUC) are known to have decreased spontaneous IgA secretion by colonic mononuclear cells. The aim of this study was to determine whether a similar alteration exists in the apparently healthy ileum of patients with CUC. The concentration of IgA was measured in the supernatant from homogenized mucosal ileal biopsies using a sandwich-type ELISA. The concentration of IgA was significantly (P=0.025) decreased in the ileum of patients with CUC (N=24) in comparison to normal ileum (N=10). The number of mucosal IgA-containing mononuclear cells (MNC) was also determined using an avidin-biotin-immunoperoxidase technique on paraffin-embedded ileal sections. Although reduced, the number of positive cells and their distribution was not significantly different in the ileum of patients with CUC (N=20) when compared to normal ileum (N=10). We suggest that decreased mucosal IgA levels are a panintestinal condition in CUC and that this is a primary alteration rather than a secondary response to the inflammatory process. Considering the role of IgA, we propose that decreased mucosal IgA levels in CUC may predispose to the disease by a reduction of the immune-mediated exclusion mechanism and/or by an impairment of the down-regulation of the inflammatory response.",
keywords = "ileum, immunoglobulin A, ulcerative colitis",
author = "Luca Cicalese and Duerr, {Richard H.} and Nalesnik, {Michael A.} and Heeckt, {Peter F.} and Lee, {Kenneth K W} and Schraut, {Wolfgang H.}",
year = "1995",
month = "4",
doi = "10.1007/BF02064983",
language = "English (US)",
volume = "40",
pages = "805--811",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis

AU - Cicalese, Luca

AU - Duerr, Richard H.

AU - Nalesnik, Michael A.

AU - Heeckt, Peter F.

AU - Lee, Kenneth K W

AU - Schraut, Wolfgang H.

PY - 1995/4

Y1 - 1995/4

N2 - Patients with chronic ulcerative colitis (CUC) are known to have decreased spontaneous IgA secretion by colonic mononuclear cells. The aim of this study was to determine whether a similar alteration exists in the apparently healthy ileum of patients with CUC. The concentration of IgA was measured in the supernatant from homogenized mucosal ileal biopsies using a sandwich-type ELISA. The concentration of IgA was significantly (P=0.025) decreased in the ileum of patients with CUC (N=24) in comparison to normal ileum (N=10). The number of mucosal IgA-containing mononuclear cells (MNC) was also determined using an avidin-biotin-immunoperoxidase technique on paraffin-embedded ileal sections. Although reduced, the number of positive cells and their distribution was not significantly different in the ileum of patients with CUC (N=20) when compared to normal ileum (N=10). We suggest that decreased mucosal IgA levels are a panintestinal condition in CUC and that this is a primary alteration rather than a secondary response to the inflammatory process. Considering the role of IgA, we propose that decreased mucosal IgA levels in CUC may predispose to the disease by a reduction of the immune-mediated exclusion mechanism and/or by an impairment of the down-regulation of the inflammatory response.

AB - Patients with chronic ulcerative colitis (CUC) are known to have decreased spontaneous IgA secretion by colonic mononuclear cells. The aim of this study was to determine whether a similar alteration exists in the apparently healthy ileum of patients with CUC. The concentration of IgA was measured in the supernatant from homogenized mucosal ileal biopsies using a sandwich-type ELISA. The concentration of IgA was significantly (P=0.025) decreased in the ileum of patients with CUC (N=24) in comparison to normal ileum (N=10). The number of mucosal IgA-containing mononuclear cells (MNC) was also determined using an avidin-biotin-immunoperoxidase technique on paraffin-embedded ileal sections. Although reduced, the number of positive cells and their distribution was not significantly different in the ileum of patients with CUC (N=20) when compared to normal ileum (N=10). We suggest that decreased mucosal IgA levels are a panintestinal condition in CUC and that this is a primary alteration rather than a secondary response to the inflammatory process. Considering the role of IgA, we propose that decreased mucosal IgA levels in CUC may predispose to the disease by a reduction of the immune-mediated exclusion mechanism and/or by an impairment of the down-regulation of the inflammatory response.

KW - ileum

KW - immunoglobulin A

KW - ulcerative colitis

UR - http://www.scopus.com/inward/record.url?scp=0028948768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028948768&partnerID=8YFLogxK

U2 - 10.1007/BF02064983

DO - 10.1007/BF02064983

M3 - Article

C2 - 7720473

AN - SCOPUS:0028948768

VL - 40

SP - 805

EP - 811

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 4

ER -