Decreased production of interleukin-10 (IL-10) by neonatal blood mononuclear leukocytes (BMLs) stimulated with tumor necrosis factor-α (TNF-α)

S. Chheda, K. H. Palkowetz, D. K. Rassin, A. S. Goldman

Research output: Contribution to journalArticle

Abstract

Background: IL-10 production by LPS or anti-CD3 stimulated newborn BMLs is greatly reduced, and TNF-α stimulates IL-10 production by adult BMLs. Since TNF-α production may be developmentally delay, we tested whether exogenous TNF-α increases IL-10 production by newborn cells. Methods: Adult and newborn BMLs were cultured with LPS and/or human recombinant TNF-α for 24h. Cell viability was tested by trypan-blue exclusion. IL-10 in supernatants from the cultures was quantified by ELISA. Results: Production of IL-10 by unstimulated adult (185 pg/ml) or newborn (121 pg/ml) BMLs was low. Mean ± SD production of IL-10 increased in adult BMLs stimulated by LPS (49111 3041 pg/ml), TNF-α alone (1520 ± 1395 pg/ml), or with LPS and TNF-α (5380 ± 3190 pg/ml), but not in newborn BMLs (LPS, 1306 ± 1216 pg/ml, p < 0.01; TNF-±, 211 ± 207 pg/ml, p < 0.01; both agents, 1225 ± 971 pg/ml, p < 0.01). Conclusions: Little IL-10 is produced by newborn BMLs in response to exogenous TNF-±. Thus, these experiments suggest that the developmental delay in IL-10 production by human newborn BMLs is not due to the paucity of intrinsic production of TNF-±, but to other mechanisms.

Original languageEnglish (US)
JournalJournal of Investigative Medicine
Volume44
Issue number1
StatePublished - 1996

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Mononuclear Leukocytes
Interleukin-10
Blood
Tumor Necrosis Factor-alpha
Trypan Blue
Cell Survival
Enzyme-Linked Immunosorbent Assay
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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Decreased production of interleukin-10 (IL-10) by neonatal blood mononuclear leukocytes (BMLs) stimulated with tumor necrosis factor-α (TNF-α). / Chheda, S.; Palkowetz, K. H.; Rassin, D. K.; Goldman, A. S.

In: Journal of Investigative Medicine, Vol. 44, No. 1, 1996.

Research output: Contribution to journalArticle

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T1 - Decreased production of interleukin-10 (IL-10) by neonatal blood mononuclear leukocytes (BMLs) stimulated with tumor necrosis factor-α (TNF-α)

AU - Chheda, S.

AU - Palkowetz, K. H.

AU - Rassin, D. K.

AU - Goldman, A. S.

PY - 1996

Y1 - 1996

N2 - Background: IL-10 production by LPS or anti-CD3 stimulated newborn BMLs is greatly reduced, and TNF-α stimulates IL-10 production by adult BMLs. Since TNF-α production may be developmentally delay, we tested whether exogenous TNF-α increases IL-10 production by newborn cells. Methods: Adult and newborn BMLs were cultured with LPS and/or human recombinant TNF-α for 24h. Cell viability was tested by trypan-blue exclusion. IL-10 in supernatants from the cultures was quantified by ELISA. Results: Production of IL-10 by unstimulated adult (185 pg/ml) or newborn (121 pg/ml) BMLs was low. Mean ± SD production of IL-10 increased in adult BMLs stimulated by LPS (49111 3041 pg/ml), TNF-α alone (1520 ± 1395 pg/ml), or with LPS and TNF-α (5380 ± 3190 pg/ml), but not in newborn BMLs (LPS, 1306 ± 1216 pg/ml, p < 0.01; TNF-±, 211 ± 207 pg/ml, p < 0.01; both agents, 1225 ± 971 pg/ml, p < 0.01). Conclusions: Little IL-10 is produced by newborn BMLs in response to exogenous TNF-±. Thus, these experiments suggest that the developmental delay in IL-10 production by human newborn BMLs is not due to the paucity of intrinsic production of TNF-±, but to other mechanisms.

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