Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since UV-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.

Original languageEnglish (US)
Pages (from-to)249-258
Number of pages10
JournalMGG Molecular & General Genetics
Volume172
Issue number3
DOIs
StatePublished - Jan 1979
Externally publishedYes

Fingerprint

DNA-Directed DNA Polymerase
Centers for Disease Control and Prevention (U.S.)
DNA Replication
Mutagenesis
Saccharomyces cerevisiae
DNA Polymerase II
DNA Polymerase I
Hot Temperature
Yeasts
Alleles
Enzymes
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae. / Prakash, Louise; Hinkle, David; Prakash, Satya.

In: MGG Molecular & General Genetics, Vol. 172, No. 3, 01.1979, p. 249-258.

Research output: Contribution to journalArticle

@article{7b84f16b3d8f429ea1a0386f7ba49b08,
title = "Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae",
abstract = "A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since UV-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.",
author = "Louise Prakash and David Hinkle and Satya Prakash",
year = "1979",
month = "1",
doi = "10.1007/BF00271724",
language = "English (US)",
volume = "172",
pages = "249--258",
journal = "Molecular and General Genetics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae

AU - Prakash, Louise

AU - Hinkle, David

AU - Prakash, Satya

PY - 1979/1

Y1 - 1979/1

N2 - A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since UV-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.

AB - A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since UV-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.

UR - http://www.scopus.com/inward/record.url?scp=0018293011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018293011&partnerID=8YFLogxK

U2 - 10.1007/BF00271724

DO - 10.1007/BF00271724

M3 - Article

VL - 172

SP - 249

EP - 258

JO - Molecular and General Genetics

JF - Molecular and General Genetics

SN - 1617-4615

IS - 3

ER -