Deep sequencing identifies noncanonical editing of Ebola and Marburg virus RNAS in infected cells

Reed S. Shabman, Omar J. Jabado, Chad Mire, Timothy B. Stockwell, Megan Edwards, Milind Mahajan, Thomas Geisbert, Christopher F. Basler

Research output: Contribution to journalArticle

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Abstract

Deep sequencing of RNAs produced by Zaire ebolavirus (EBOV) or the Angola strain of Marburgvirus (MARV-Ang) identified novel viral and cellular mechanisms that diversify the coding and noncoding sequences of viral mRNAs and genomic RNAs. We identified previously undescribed sites within the EBOV and MARV-Ang mRNAs where apparent cotranscriptional editing has resulted in the addition of non-template-encoded residues within the EBOV glycoprotein (GP) mRNA, the MARVAng nucleoprotein (NP) mRNA, and the MARV-Ang polymerase (L) mRNA, such that novel viral translation products could be produced. Further, we found that the well-characterized EBOV GP mRNA editing site is modified at a high frequency during viral genome RNA replication. Additionally, editing hot spots representing sites of apparent adenosine deaminase activity were found in the MARV-Ang NP 3=-untranslated region. These studies identify novel filovirus-host interactions and reveal production of a greater diversity of filoviral gene products than was previously appreciated.

Original languageEnglish (US)
Article numbere02011-14
JournalmBio
Volume5
Issue number6
DOIs
StatePublished - Sep 25 2014

Fingerprint

Marburgvirus
Ebolavirus
High-Throughput Nucleotide Sequencing
Messenger RNA
Nucleoproteins
Glycoproteins
Angola
RNA
Democratic Republic of the Congo
Adenosine Deaminase
Viral Genome
Viral RNA
3' Untranslated Regions

ASJC Scopus subject areas

  • Microbiology
  • Virology

Cite this

Shabman, R. S., Jabado, O. J., Mire, C., Stockwell, T. B., Edwards, M., Mahajan, M., ... Basler, C. F. (2014). Deep sequencing identifies noncanonical editing of Ebola and Marburg virus RNAS in infected cells. mBio, 5(6), [e02011-14]. https://doi.org/10.1128/mBio.02011-14.

Deep sequencing identifies noncanonical editing of Ebola and Marburg virus RNAS in infected cells. / Shabman, Reed S.; Jabado, Omar J.; Mire, Chad; Stockwell, Timothy B.; Edwards, Megan; Mahajan, Milind; Geisbert, Thomas; Basler, Christopher F.

In: mBio, Vol. 5, No. 6, e02011-14, 25.09.2014.

Research output: Contribution to journalArticle

Shabman, RS, Jabado, OJ, Mire, C, Stockwell, TB, Edwards, M, Mahajan, M, Geisbert, T & Basler, CF 2014, 'Deep sequencing identifies noncanonical editing of Ebola and Marburg virus RNAS in infected cells', mBio, vol. 5, no. 6, e02011-14. https://doi.org/10.1128/mBio.02011-14.
Shabman RS, Jabado OJ, Mire C, Stockwell TB, Edwards M, Mahajan M et al. Deep sequencing identifies noncanonical editing of Ebola and Marburg virus RNAS in infected cells. mBio. 2014 Sep 25;5(6). e02011-14. https://doi.org/10.1128/mBio.02011-14.
Shabman, Reed S. ; Jabado, Omar J. ; Mire, Chad ; Stockwell, Timothy B. ; Edwards, Megan ; Mahajan, Milind ; Geisbert, Thomas ; Basler, Christopher F. / Deep sequencing identifies noncanonical editing of Ebola and Marburg virus RNAS in infected cells. In: mBio. 2014 ; Vol. 5, No. 6.
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