Defective DNA endonuclease activity on anthramycin treated DNA in xeroderma pigmentosum and mouse melanoma cells

Dwight E. Lee, Anthony O. Okorodudu, W. Clark Lambert, Muriel W. Lambert

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Nine separate DNA endonuclease activities from non-histone chromatin proteins and a corresponding set from the nucleoplasm of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid and Cloudman mouse melanoma cells, obtained by isoelectric focusing, were tested against circular duplex phage PM2 DNA previously treated with anthramycin. A marked increase in activity against anthramycin treated DNA was found in normal human lymphoblastoid cells in a chromatin fraction with pI 4.6, with lesser increases at pI's 3.9 and 5.4 and a nucleoplasmic fraction at pI 4.6. In the nuclear proteins of xeroderma pigmentosum and mouse melanoma cells, however, no increase in activity against anthramycin DNA could be detected in any fraction.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume107
Issue number1
DOIs
StatePublished - Jul 16 1982
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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