TY - JOUR
T1 - Defective DNA endonuclease activity on anthramycin treated DNA in xeroderma pigmentosum and mouse melanoma cells
AU - Lee, Dwight E.
AU - Okorodudu, Anthony O.
AU - Lambert, W. Clark
AU - Lambert, Muriel W.
PY - 1982/7/16
Y1 - 1982/7/16
N2 - Nine separate DNA endonuclease activities from non-histone chromatin proteins and a corresponding set from the nucleoplasm of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid and Cloudman mouse melanoma cells, obtained by isoelectric focusing, were tested against circular duplex phage PM2 DNA previously treated with anthramycin. A marked increase in activity against anthramycin treated DNA was found in normal human lymphoblastoid cells in a chromatin fraction with pI 4.6, with lesser increases at pI's 3.9 and 5.4 and a nucleoplasmic fraction at pI 4.6. In the nuclear proteins of xeroderma pigmentosum and mouse melanoma cells, however, no increase in activity against anthramycin DNA could be detected in any fraction.
AB - Nine separate DNA endonuclease activities from non-histone chromatin proteins and a corresponding set from the nucleoplasm of normal human and xeroderma pigmentosum, complementation group A, lymphoblastoid and Cloudman mouse melanoma cells, obtained by isoelectric focusing, were tested against circular duplex phage PM2 DNA previously treated with anthramycin. A marked increase in activity against anthramycin treated DNA was found in normal human lymphoblastoid cells in a chromatin fraction with pI 4.6, with lesser increases at pI's 3.9 and 5.4 and a nucleoplasmic fraction at pI 4.6. In the nuclear proteins of xeroderma pigmentosum and mouse melanoma cells, however, no increase in activity against anthramycin DNA could be detected in any fraction.
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U2 - 10.1016/0006-291X(82)91717-X
DO - 10.1016/0006-291X(82)91717-X
M3 - Article
C2 - 6215036
AN - SCOPUS:0020456516
SN - 0006-291X
VL - 107
SP - 395
EP - 402
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -