Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae

Raymond D. Miller, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalMGG Molecular & General Genetics
Volume188
Issue number2
DOIs
StatePublished - Dec 1982
Externally publishedYes

Fingerprint

Pyrimidine Dimers
Saccharomyces cerevisiae
DNA
Chromatin
Mutation
Enzymes
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

@article{c30d18159d524d3f9cabccc4aa200933,
title = "Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae",
abstract = "Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60{\%} of pyrimidine dimers and 25-40{\%} of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.",
author = "Miller, {Raymond D.} and Louise Prakash and Satya Prakash",
year = "1982",
month = "12",
doi = "10.1007/BF00332681",
language = "English (US)",
volume = "188",
pages = "235--239",
journal = "Molecular and General Genetics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Defective excision of pyrimidine dimers and interstrand DNA crosslinks in rad7 and rad23 mutants of Saccharomyces cerevisiae

AU - Miller, Raymond D.

AU - Prakash, Louise

AU - Prakash, Satya

PY - 1982/12

Y1 - 1982/12

N2 - Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

AB - Excision of pyrimidine dimers and interstrand DNA crosslinks was examined in the deletion mutants rad7-Δ1, rad23-Δ1, and rad7-Δ1 rad23-Δ1. These mutants remove pyrimidine dimers and crosslinks much less efficiently than the RAD+ strains; only 30-60% of pyrimidine dimers and 25-40% of crosslinks are removed even after prolonged incubation. The rad7 and rad23 mutations may represent defects in protein factors which increase the efficiency of the nicking enzyme complex or make chromatin more accessible to the nicking activity.

UR - http://www.scopus.com/inward/record.url?scp=0020357694&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020357694&partnerID=8YFLogxK

U2 - 10.1007/BF00332681

DO - 10.1007/BF00332681

M3 - Article

VL - 188

SP - 235

EP - 239

JO - Molecular and General Genetics

JF - Molecular and General Genetics

SN - 1617-4615

IS - 2

ER -