Deficient T-cell mitogen response in murine experimental autoimmune myasthenia gravis: A defect in the adherent cell population

Premkumar Christadoss, Michael Dauphinee, Jon Lindstrom, Howard Dang, Gabriel Fernandes, Norman Talal

Research output: Contribution to journalArticle

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Abstract

T-Lymphocyte number and functions are often reduced, while B-lymphocyte function is often increased in patients with autoimmune disorders. To study the mechanisms responsible for these T-cell malfunctions in autoimmunity we adapted the murine experimental autoimmune myasthenia gravis (EAMG) model. Splenocytes from C57BL/6 mice immunized with acetylcholine receptors (AChR) in complete Freund's adjuvant (CFA) produced approximately half the amount of concanavalin A (Con A)-induced interleukin 2 (IL-2) as did splenocytes of CFA-inoculated controls. Further, AChR plus CFA-immunized splenocytes showed a marked reduction in T-cell proliferative responses induced by Con A or phytohemagglutinin when compared with CFA-inoculated controls. By contrast, lipopolysaccharide-induced B-cell function is preserved. Deficient Con A splenic T-cell response is seen early after secondary inoculation with CFA or AChR in CFA. T-Cell recovery occurs in CFA-inoculated mice but not in AChR plus CFA-inoculated mice. Defective Con A splenic T-cell response seen early after secondary immunization with CFA or AChR in CFA is due to the presence of a defective splenic adherent cell population. Moreover, defective Con A splenic T-cell response seen after established autoimmunity to AChR in EAMG is also due to the presence of a defective splenic adherent cell population.

Original languageEnglish (US)
Pages (from-to)358-366
Number of pages9
JournalCellular Immunology
Volume79
Issue number2
DOIs
StatePublished - Jul 15 1983
Externally publishedYes

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Autoimmune Experimental Myasthenia Gravis
Freund's Adjuvant
Mitogens
T-Lymphocytes
Cholinergic Receptors
Concanavalin A
Population
Autoimmunity
B-Lymphocytes
Secondary Immunization
Lymphocyte Count
Phytohemagglutinins
Inbred C57BL Mouse
Interleukin-2

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Deficient T-cell mitogen response in murine experimental autoimmune myasthenia gravis : A defect in the adherent cell population. / Christadoss, Premkumar; Dauphinee, Michael; Lindstrom, Jon; Dang, Howard; Fernandes, Gabriel; Talal, Norman.

In: Cellular Immunology, Vol. 79, No. 2, 15.07.1983, p. 358-366.

Research output: Contribution to journalArticle

Christadoss, Premkumar ; Dauphinee, Michael ; Lindstrom, Jon ; Dang, Howard ; Fernandes, Gabriel ; Talal, Norman. / Deficient T-cell mitogen response in murine experimental autoimmune myasthenia gravis : A defect in the adherent cell population. In: Cellular Immunology. 1983 ; Vol. 79, No. 2. pp. 358-366.
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