TY - JOUR
T1 - Defining Molecular and Domain Boundaries in the Bacteriophage ϕ29 DNA Packaging Motor
AU - Morais, Marc C.
AU - Koti, Jaya S.
AU - Bowman, Valorie D.
AU - Reyes-Aldrete, Emilio
AU - Anderson, Dwight L.
AU - Rossmann, Michael G.
PY - 2008/8/6
Y1 - 2008/8/6
N2 - Cryo-electron microscopy (cryo-EM) studies of the bacteriophage φ{symbol}29 DNA packaging motor have delineated the relative positions and molecular boundaries of the 12-fold symmetric head-tail connector, the 5-fold symmetric prohead RNA (pRNA), the ATPase that provides the energy for packaging, and the procapsid. Reconstructions, assuming 5-fold symmetry, were determined for proheads with 174-base, 120-base, and 71-base pRNA; proheads lacking pRNA; proheads with ATPase bound; and proheads in which the packaging motor was missing the connector. These structures are consistent with pRNA and ATPase forming a pentameric motor component around the unique vertex of proheads. They suggest an assembly pathway for the packaging motor and a mechanism for DNA translocation into empty proheads.
AB - Cryo-electron microscopy (cryo-EM) studies of the bacteriophage φ{symbol}29 DNA packaging motor have delineated the relative positions and molecular boundaries of the 12-fold symmetric head-tail connector, the 5-fold symmetric prohead RNA (pRNA), the ATPase that provides the energy for packaging, and the procapsid. Reconstructions, assuming 5-fold symmetry, were determined for proheads with 174-base, 120-base, and 71-base pRNA; proheads lacking pRNA; proheads with ATPase bound; and proheads in which the packaging motor was missing the connector. These structures are consistent with pRNA and ATPase forming a pentameric motor component around the unique vertex of proheads. They suggest an assembly pathway for the packaging motor and a mechanism for DNA translocation into empty proheads.
KW - MICROBIO
KW - PROTEIN
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U2 - 10.1016/j.str.2008.05.010
DO - 10.1016/j.str.2008.05.010
M3 - Article
C2 - 18682228
VL - 16
SP - 1267
EP - 1274
JO - Structure with Folding & design
JF - Structure with Folding & design
SN - 0969-2126
IS - 8
ER -