Definition of a functional single nucleotide polymorphism in the cell migration inhibitory Gene MIIP that affects the risk of breast cancer

Fangfang Song, Ping Ji, Hong Zheng, Fengju Song, Yingmei Wang, Xishan Hao, Qingyi Wei, Wei Zhang, Kexin Chen

Research output: Contribution to journalArticle

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Abstract

The migration and invasion inhibitory protein MIIP is an inhibitor of cancer cell migration and invasion that inhibits breast tumorigenesis. In this case-control study, we evaluated the MIIP single nucleotide polymorphism (SNP) rs2295283 (codon 167, A>G, K>E) from 1,524 breast cancer patients and 1,592 age-matched controls for its association with breast cancer risk. SNP analysis included a validation set of 736 cases and 760 controls. Colony formation and cell migration assays were then conducted to functionally interrogate the genotype difference. When compared with the AA genotype, the combined AG + GG genotypes (167E) were associated with a significantly lower risk of breast cancer. In the test set, the protective effects of the AG + GG genotypes were more evident among participants with a family history of cancer. Further case series analysis revealed that the GG genotype was associated with reduced breast cancer susceptibility in cases of tumor size >2 cm and late clinical stage (II + III + IV). Colony formation assays showed that MIIP 167E (the G variant) was a more potent inhibitor of colony formation but not cell migration. These results suggest MIIP K167E as a functional genetic marker of breast cancer development and prognosis.

Original languageEnglish (US)
Pages (from-to)1024-1032
Number of pages9
JournalCancer Research
Volume70
Issue number3
DOIs
StatePublished - Feb 1 2010
Externally publishedYes

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Cell Movement
Single Nucleotide Polymorphism
Genotype
Breast Neoplasms
Genes
Cell Migration Assays
Neoplasms
Genetic Markers
Codon
Case-Control Studies
Carcinogenesis
Breast
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Definition of a functional single nucleotide polymorphism in the cell migration inhibitory Gene MIIP that affects the risk of breast cancer. / Song, Fangfang; Ji, Ping; Zheng, Hong; Song, Fengju; Wang, Yingmei; Hao, Xishan; Wei, Qingyi; Zhang, Wei; Chen, Kexin.

In: Cancer Research, Vol. 70, No. 3, 01.02.2010, p. 1024-1032.

Research output: Contribution to journalArticle

Song, Fangfang ; Ji, Ping ; Zheng, Hong ; Song, Fengju ; Wang, Yingmei ; Hao, Xishan ; Wei, Qingyi ; Zhang, Wei ; Chen, Kexin. / Definition of a functional single nucleotide polymorphism in the cell migration inhibitory Gene MIIP that affects the risk of breast cancer. In: Cancer Research. 2010 ; Vol. 70, No. 3. pp. 1024-1032.
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