Degranulation of uterine mast cell modifies contractility of isolated myometrium from pregnant women

Egle Bytautiene, Yuri P. Vedernikov, George Saade, Roberto Romero, Robert E. Garfield

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

This study was undertaken to test that uterine mast cell degranulation alters human myometrial contractility in vitro and to define what mediators are involved in this process. Longitudinal myometrial strips prepared from biopsy specimen obtained from the lower uterine segment of women at preterm and term gestation (with and without labor) were studied. Contractile responses to compound 48/80, a mast cell degranulator, were compared in the absence or presence of a mast cell stabilizer, H 1 and H 2 receptor antagonists, cyclooxygenase, and lipoxygenase inhibitors. Compound 48/80 increased myometrial contractility in all groups. The mast cell stabilizer cromolyn inhibited contractility, whereas the cyclooxygenase inhibitor ibuprofen, the H 1-receptor antagonist S(+)-chlorpheniramine maleate, but not the H 2 antagonist cimetidine, only slightly attenuated this effect. The lipoxygenase inhibitor linoleyl hydroxamic acid augmented the responses to compound 48/80 in the preterm but not in the term group. Uterine mast cell degranulation, or the effects of their mediators, can modulate uterine contractility during pregnancy.

Original languageEnglish
Pages (from-to)1705-1710
Number of pages6
JournalAmerican Journal of Obstetrics and Gynecology
Volume191
Issue number5
DOIs
StatePublished - Nov 2004

Fingerprint

Myometrium
Mast Cells
Pregnant Women
p-Methoxy-N-methylphenethylamine
Cell Degranulation
Lipoxygenase Inhibitors
Cyclooxygenase Inhibitors
Chlorpheniramine
Pregnancy
Cromolyn Sodium
Ibuprofen
Cimetidine

Keywords

  • Antihistaminic
  • Cromolyn sodium
  • Human pregnancy
  • Mast cells
  • Uterine contractility

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Degranulation of uterine mast cell modifies contractility of isolated myometrium from pregnant women. / Bytautiene, Egle; Vedernikov, Yuri P.; Saade, George; Romero, Roberto; Garfield, Robert E.

In: American Journal of Obstetrics and Gynecology, Vol. 191, No. 5, 11.2004, p. 1705-1710.

Research output: Contribution to journalArticle

Bytautiene, Egle ; Vedernikov, Yuri P. ; Saade, George ; Romero, Roberto ; Garfield, Robert E. / Degranulation of uterine mast cell modifies contractility of isolated myometrium from pregnant women. In: American Journal of Obstetrics and Gynecology. 2004 ; Vol. 191, No. 5. pp. 1705-1710.
@article{f63a285e9e1649dcaae72eb0e5549626,
title = "Degranulation of uterine mast cell modifies contractility of isolated myometrium from pregnant women",
abstract = "This study was undertaken to test that uterine mast cell degranulation alters human myometrial contractility in vitro and to define what mediators are involved in this process. Longitudinal myometrial strips prepared from biopsy specimen obtained from the lower uterine segment of women at preterm and term gestation (with and without labor) were studied. Contractile responses to compound 48/80, a mast cell degranulator, were compared in the absence or presence of a mast cell stabilizer, H 1 and H 2 receptor antagonists, cyclooxygenase, and lipoxygenase inhibitors. Compound 48/80 increased myometrial contractility in all groups. The mast cell stabilizer cromolyn inhibited contractility, whereas the cyclooxygenase inhibitor ibuprofen, the H 1-receptor antagonist S(+)-chlorpheniramine maleate, but not the H 2 antagonist cimetidine, only slightly attenuated this effect. The lipoxygenase inhibitor linoleyl hydroxamic acid augmented the responses to compound 48/80 in the preterm but not in the term group. Uterine mast cell degranulation, or the effects of their mediators, can modulate uterine contractility during pregnancy.",
keywords = "Antihistaminic, Cromolyn sodium, Human pregnancy, Mast cells, Uterine contractility",
author = "Egle Bytautiene and Vedernikov, {Yuri P.} and George Saade and Roberto Romero and Garfield, {Robert E.}",
year = "2004",
month = "11",
doi = "10.1016/j.ajog.2004.04.008",
language = "English",
volume = "191",
pages = "1705--1710",
journal = "American Journal of Obstetrics and Gynecology",
issn = "0002-9378",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Degranulation of uterine mast cell modifies contractility of isolated myometrium from pregnant women

AU - Bytautiene, Egle

AU - Vedernikov, Yuri P.

AU - Saade, George

AU - Romero, Roberto

AU - Garfield, Robert E.

PY - 2004/11

Y1 - 2004/11

N2 - This study was undertaken to test that uterine mast cell degranulation alters human myometrial contractility in vitro and to define what mediators are involved in this process. Longitudinal myometrial strips prepared from biopsy specimen obtained from the lower uterine segment of women at preterm and term gestation (with and without labor) were studied. Contractile responses to compound 48/80, a mast cell degranulator, were compared in the absence or presence of a mast cell stabilizer, H 1 and H 2 receptor antagonists, cyclooxygenase, and lipoxygenase inhibitors. Compound 48/80 increased myometrial contractility in all groups. The mast cell stabilizer cromolyn inhibited contractility, whereas the cyclooxygenase inhibitor ibuprofen, the H 1-receptor antagonist S(+)-chlorpheniramine maleate, but not the H 2 antagonist cimetidine, only slightly attenuated this effect. The lipoxygenase inhibitor linoleyl hydroxamic acid augmented the responses to compound 48/80 in the preterm but not in the term group. Uterine mast cell degranulation, or the effects of their mediators, can modulate uterine contractility during pregnancy.

AB - This study was undertaken to test that uterine mast cell degranulation alters human myometrial contractility in vitro and to define what mediators are involved in this process. Longitudinal myometrial strips prepared from biopsy specimen obtained from the lower uterine segment of women at preterm and term gestation (with and without labor) were studied. Contractile responses to compound 48/80, a mast cell degranulator, were compared in the absence or presence of a mast cell stabilizer, H 1 and H 2 receptor antagonists, cyclooxygenase, and lipoxygenase inhibitors. Compound 48/80 increased myometrial contractility in all groups. The mast cell stabilizer cromolyn inhibited contractility, whereas the cyclooxygenase inhibitor ibuprofen, the H 1-receptor antagonist S(+)-chlorpheniramine maleate, but not the H 2 antagonist cimetidine, only slightly attenuated this effect. The lipoxygenase inhibitor linoleyl hydroxamic acid augmented the responses to compound 48/80 in the preterm but not in the term group. Uterine mast cell degranulation, or the effects of their mediators, can modulate uterine contractility during pregnancy.

KW - Antihistaminic

KW - Cromolyn sodium

KW - Human pregnancy

KW - Mast cells

KW - Uterine contractility

UR - http://www.scopus.com/inward/record.url?scp=8544232112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8544232112&partnerID=8YFLogxK

U2 - 10.1016/j.ajog.2004.04.008

DO - 10.1016/j.ajog.2004.04.008

M3 - Article

C2 - 15547545

AN - SCOPUS:8544232112

VL - 191

SP - 1705

EP - 1710

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

SN - 0002-9378

IS - 5

ER -