Delayed start-up of kinesin-driven microtubule gliding following inhibition by adenosine 5′-[β,γ-imido]triphosphate

Bruch J. Schnapp, Bruce Crise, Michael Sheetz, Thomas S. Reese, Shahid Khan

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Kinesin is a microtubule-activated ATPase that moves objects toward the plus end of microtubules and makes microtubules glide along a glass surface. Here we investigate a remarkable effect of the nonhydrolyzable analogue of ATP, adenosine 5′-[β,γ-imido]triphosphate (p[NH]ppA), on kinesin-driven microtubule gliding. Microtubule gliding that has been blocked by rapid replacement of ATP with p[NH]ppA requires 1-2 min of exposure to ATP before microtubule gliding resumes. This latency is not shortened by prolonged washing of p[NH]ppA-blocked microtubules in nucleotide-free buffer for up to 15 min, suggesting that ATP binding to a second nucleotide binding site on kinesin triggers the release of bound p[NH]ppA. To test this hypothesis, the release of [3H]p[NH]ppA from kinesin-microtubule complexes was followed in parallel biochemical assays. In nucleotide-free buffer, the bound p[NH]ppA was released over several hours from the complexes. However, addition of ATP caused the release of p[NH]ppA from the ktaesin-microtubule complexes within 2 min, which was similar to the latent period for start-up of microtubule gliding after p[NH]ppA inhibition. The stoichiometry of p[NH]ppA bound per kinesin heavy chain at saturation was estimated to be ≈ 1:2, These results suggest a model in which each molecule of kinesin has at least two nucleotide binding sites that alternately bind nucleotide.

Original languageEnglish (US)
Pages (from-to)10053-10057
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number24
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

Kinesin
Microtubules
Adenosine
Nucleotides
Adenosine Triphosphate
Buffers
Binding Sites
triphosphoric acid
Glass
Adenosine Triphosphatases

Keywords

  • Fast axonal transport
  • Motor proteins

ASJC Scopus subject areas

  • General

Cite this

Delayed start-up of kinesin-driven microtubule gliding following inhibition by adenosine 5′-[β,γ-imido]triphosphate. / Schnapp, Bruch J.; Crise, Bruce; Sheetz, Michael; Reese, Thomas S.; Khan, Shahid.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 24, 01.01.1990, p. 10053-10057.

Research output: Contribution to journalArticle

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