Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs

Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kobayashi, Yumi Kirino, Tamaki Okabayashi, Nariaki Nonaka, Hirohisa Mekata, Hiroshi Aoki, Mai Shiokawa, Moeko Umetsu, Tatsushi Morita, Ayako Hasebe, Keiko Otsu, Tetsuo AsaiTomohiro Yamaguchi, Shinji Makino, Yoshiteru Murata, Ahmad Jan Abi, Tsutomu Omatsu, Tetsuya Mizutani

Research output: Contribution to journalArticle

Abstract

Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.

Original languageEnglish (US)
Pages (from-to)350-357
Number of pages8
JournalJournal of Veterinary Science
Volume19
Issue number3
DOIs
StatePublished - Jan 1 2018

Fingerprint

vector competence
abortion (animals)
Bovine Enterovirus
respiratory tract diseases
Neospora
Bovine Viral Diarrhea Viruses
Communicable Diseases
Diarrhea
diarrhea
Salmonella enterica
polymerase chain reaction
Diptera
Polymerase Chain Reaction
Birds
Insects
pathogens
Rodentia
cattle
Cockroaches
Bovine enterovirus

Keywords

  • Cattle
  • Dembo polymerase chain reaction
  • Disease reservoirs
  • Disease vectors
  • Virulence factors

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs. / Rahpaya, Sayed Samim; Tsuchiaka, Shinobu; Kishimoto, Mai; Oba, Mami; Katayama, Yukie; Nunomura, Yuka; Kokawa, Saki; Kimura, Takashi; Kobayashi, Atsushi; Kirino, Yumi; Okabayashi, Tamaki; Nonaka, Nariaki; Mekata, Hirohisa; Aoki, Hiroshi; Shiokawa, Mai; Umetsu, Moeko; Morita, Tatsushi; Hasebe, Ayako; Otsu, Keiko; Asai, Tetsuo; Yamaguchi, Tomohiro; Makino, Shinji; Murata, Yoshiteru; Abi, Ahmad Jan; Omatsu, Tsutomu; Mizutani, Tetsuya.

In: Journal of Veterinary Science, Vol. 19, No. 3, 01.01.2018, p. 350-357.

Research output: Contribution to journalArticle

Rahpaya, SS, Tsuchiaka, S, Kishimoto, M, Oba, M, Katayama, Y, Nunomura, Y, Kokawa, S, Kimura, T, Kobayashi, A, Kirino, Y, Okabayashi, T, Nonaka, N, Mekata, H, Aoki, H, Shiokawa, M, Umetsu, M, Morita, T, Hasebe, A, Otsu, K, Asai, T, Yamaguchi, T, Makino, S, Murata, Y, Abi, AJ, Omatsu, T & Mizutani, T 2018, 'Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs', Journal of Veterinary Science, vol. 19, no. 3, pp. 350-357. https://doi.org/10.4142/jvs.2018.19.3.350
Rahpaya, Sayed Samim ; Tsuchiaka, Shinobu ; Kishimoto, Mai ; Oba, Mami ; Katayama, Yukie ; Nunomura, Yuka ; Kokawa, Saki ; Kimura, Takashi ; Kobayashi, Atsushi ; Kirino, Yumi ; Okabayashi, Tamaki ; Nonaka, Nariaki ; Mekata, Hirohisa ; Aoki, Hiroshi ; Shiokawa, Mai ; Umetsu, Moeko ; Morita, Tatsushi ; Hasebe, Ayako ; Otsu, Keiko ; Asai, Tetsuo ; Yamaguchi, Tomohiro ; Makino, Shinji ; Murata, Yoshiteru ; Abi, Ahmad Jan ; Omatsu, Tsutomu ; Mizutani, Tetsuya. / Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs. In: Journal of Veterinary Science. 2018 ; Vol. 19, No. 3. pp. 350-357.
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AU - Kobayashi, Atsushi

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AU - Nonaka, Nariaki

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AU - Aoki, Hiroshi

AU - Shiokawa, Mai

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AU - Morita, Tatsushi

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