Dendritic glutamate release produces autocrine activation of mGluR1 in cerebellar Purkinje cells

Hoon Shin Jung, Yu Shin Kim, David J. Linden

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In recent years, it has become clear that, in addition to conventional anterograde transmission, signaling in neural circuits can occur in a retrograde manner. This suggests the additional possibility that postsynaptic release of neurotransmitter might be able to act in an autocrine fashion. Here, we show that brief depolarization of a cerebellar Purkinje cell triggers a slow inward current. This depolarization-induced slow current (DISC) is attenuated by antagonists of mGluR1 or TRP channels. DISC is eliminated by a mixture of voltage-sensitive Ca2+ channel blockers and is mimicked by a brief climbing fiber burst. DISC is attenuated by an inhibitor of vesicular glutamate transporters or of vesicular fusion. These data suggest that Ca 2+-dependent postsynaptic fusion of glutamate-loaded vesicles evokes a slow inward current produced by activation of postsynaptic mGluR1, thereby constituting a useful form of feedback regulation.

Original languageEnglish (US)
Pages (from-to)746-750
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number2
DOIs
StatePublished - Jan 15 2008
Externally publishedYes

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Purkinje Cells
Glutamic Acid
Vesicular Glutamate Transport Proteins
Neurotransmitter Agents
metabotropic glutamate receptor type 1

Keywords

  • Ca channel
  • Retrograde signaling
  • Vesicular fusion

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Dendritic glutamate release produces autocrine activation of mGluR1 in cerebellar Purkinje cells. / Jung, Hoon Shin; Kim, Yu Shin; Linden, David J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 2, 15.01.2008, p. 746-750.

Research output: Contribution to journalArticle

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