Dendro[C 60]fullerene DF-1 provides radioprotection to radiosensitive mammalian cells

Corey A. Theriot, Rachael C. Casey, Valerie C. Moore, Linsey Mitchell, Julia O. Reynolds, Madeline Burgoyne, Ranga Partha, Janice L. Huff, Jodie L. Conyers, Antony Jeevarajan, Honglu Wu

    Research output: Contribution to journalArticlepeer-review

    36 Scopus citations


    In this study, the ability of the C 60 fullerene derivative DF-1 to protect radiosensitive cells from the effects of high doses of gamma irradiation was examined. Earlier reports of DF-1's lack of toxicity in these cells were confirmed, and DF-1 was also observed to protect both human lymphocytes and rat intestinal crypt cells against radiation-induced cell death. We determined that DF-1 protected both cell types against radiation-induced DNA damage, as measured by inhibition of micronucleus formation. DF-1 also reduced the levels of reactive oxygen species in the crypt cells, a unique capability of fullerenes because of their enhanced reactivity toward electron-rich species. The ability of DF-1 to protect against the cytotoxic effects of radiation was comparable to that of amifostine, another ROS-scavenging radioprotector. Interestingly, localization of fluorescently labeled DF-1 in fibroblast was observed throughout the cell. Taken together, these results suggest that DF-1 provides powerful protection against several deleterious cellular consequences of irradiation in mammalian systems including oxidative stress, DNA damage, and cell death.

    Original languageEnglish (US)
    Pages (from-to)437-445
    Number of pages9
    JournalRadiation and Environmental Biophysics
    Issue number3
    StatePublished - Aug 2010

    ASJC Scopus subject areas

    • Biophysics
    • Radiation
    • General Environmental Science


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