Dengue virus binding to human leukocyte cell lines

Receptor usage differs between cell types and virus strains

Helle Bielefeldt-Ohmann, Michelle Meyer, David R. Fitzpatrick, John S. Mackenzie

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Monocyte macrophages (Mψ) are thought to be the principal target cells for the dengue viruses (DV), the cause of dengue fever and hemorrhagic fever. Cell attachment is mediated by the virus envelope (E) protein, but the host-cell receptors remain elusive. Currently, candidate receptor molecules include proteins, Fc receptors, glycosaminoglycans (GAGs) and lipopolysaccharide binding CD14-associated molecules. Here, we show that in addition to Mψ, cells of the T- and B-cell lineages, and including cells lacking GAGs, can bind and become infected with DV. The level of virus binding varied widely between cell lines and, notably, between virus strains within a DV serotype. The latter difference may be ascribable to one or more amino acid differences in domain II of the E protein. Heparin had no significant effect on DV binding, while heparinase treatment of cells in all cases increased DV binding, further supporting the contention that GAGs are not required for DV binding and infection of human cells. In contrast to a recent report, we found that lipopolysaccharide (LPS) had either no effect or enhanced DV binding to, and infection of, various human leukocyte cell lines, while in all virus-cell combinations, depletion of Ca2+/Mg2+ enhanced DV binding. This argues against involvement of β2 integrins in virus-host cell interactions, a conclusion in accord with the demonstration of three virus binding membrane proteins of

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalVirus Research
Volume73
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Virus Attachment
Dengue Virus
Leukocytes
Viruses
Cell Line
Glycosaminoglycans
Virus Diseases
Lipopolysaccharides
Heparin Lyase
Viral Envelope Proteins
Severe Dengue
Fc Receptors
Cell Lineage
Integrins
Cell Communication
Heparin
Monocytes
Carrier Proteins
Membrane Proteins
Proteins

Keywords

  • β2 integrin
  • Dengue virus
  • Glycosaminoglycans
  • Host cell receptor

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Virology

Cite this

Dengue virus binding to human leukocyte cell lines : Receptor usage differs between cell types and virus strains. / Bielefeldt-Ohmann, Helle; Meyer, Michelle; Fitzpatrick, David R.; Mackenzie, John S.

In: Virus Research, Vol. 73, No. 1, 2001, p. 81-89.

Research output: Contribution to journalArticle

Bielefeldt-Ohmann, Helle ; Meyer, Michelle ; Fitzpatrick, David R. ; Mackenzie, John S. / Dengue virus binding to human leukocyte cell lines : Receptor usage differs between cell types and virus strains. In: Virus Research. 2001 ; Vol. 73, No. 1. pp. 81-89.
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