Dengue virus NS1 protein interacts with the ribosomal protein RPL18: This interaction is required for viral translation and replication in Huh-7 cells

Margot Cervantes-Salazar, Antonio H. Angel-Ambrocio, Ruben Soto-Acosta, Patricia Bautista-Carbajal, Arianna M. Hurtado-Monzon, Sofia L. Alcaraz-Estrada, Juan E. Ludert, Rosa M. Del Angel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48. hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.

Original languageEnglish (US)
Pages (from-to)113-126
Number of pages14
JournalVirology
Volume484
DOIs
StatePublished - Oct 1 2015
Externally publishedYes

Fingerprint

Dengue Virus
Ribosomal Proteins
Proteins
Genome
Cellular Structures
Infection
Affinity Chromatography
Immunoprecipitation
Virion
Hepatocytes

Keywords

  • Dengue
  • NS1
  • Replication
  • Ribosomal proteins

ASJC Scopus subject areas

  • Virology

Cite this

Cervantes-Salazar, M., Angel-Ambrocio, A. H., Soto-Acosta, R., Bautista-Carbajal, P., Hurtado-Monzon, A. M., Alcaraz-Estrada, S. L., ... Del Angel, R. M. (2015). Dengue virus NS1 protein interacts with the ribosomal protein RPL18: This interaction is required for viral translation and replication in Huh-7 cells. Virology, 484, 113-126. https://doi.org/10.1016/j.virol.2015.05.017

Dengue virus NS1 protein interacts with the ribosomal protein RPL18 : This interaction is required for viral translation and replication in Huh-7 cells. / Cervantes-Salazar, Margot; Angel-Ambrocio, Antonio H.; Soto-Acosta, Ruben; Bautista-Carbajal, Patricia; Hurtado-Monzon, Arianna M.; Alcaraz-Estrada, Sofia L.; Ludert, Juan E.; Del Angel, Rosa M.

In: Virology, Vol. 484, 01.10.2015, p. 113-126.

Research output: Contribution to journalArticle

Cervantes-Salazar, M, Angel-Ambrocio, AH, Soto-Acosta, R, Bautista-Carbajal, P, Hurtado-Monzon, AM, Alcaraz-Estrada, SL, Ludert, JE & Del Angel, RM 2015, 'Dengue virus NS1 protein interacts with the ribosomal protein RPL18: This interaction is required for viral translation and replication in Huh-7 cells', Virology, vol. 484, pp. 113-126. https://doi.org/10.1016/j.virol.2015.05.017
Cervantes-Salazar M, Angel-Ambrocio AH, Soto-Acosta R, Bautista-Carbajal P, Hurtado-Monzon AM, Alcaraz-Estrada SL et al. Dengue virus NS1 protein interacts with the ribosomal protein RPL18: This interaction is required for viral translation and replication in Huh-7 cells. Virology. 2015 Oct 1;484:113-126. https://doi.org/10.1016/j.virol.2015.05.017
Cervantes-Salazar, Margot ; Angel-Ambrocio, Antonio H. ; Soto-Acosta, Ruben ; Bautista-Carbajal, Patricia ; Hurtado-Monzon, Arianna M. ; Alcaraz-Estrada, Sofia L. ; Ludert, Juan E. ; Del Angel, Rosa M. / Dengue virus NS1 protein interacts with the ribosomal protein RPL18 : This interaction is required for viral translation and replication in Huh-7 cells. In: Virology. 2015 ; Vol. 484. pp. 113-126.
@article{ae1addd1353246cc93cd31b6794641f2,
title = "Dengue virus NS1 protein interacts with the ribosomal protein RPL18: This interaction is required for viral translation and replication in Huh-7 cells",
abstract = "Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48. hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.",
keywords = "Dengue, NS1, Replication, Ribosomal proteins",
author = "Margot Cervantes-Salazar and Angel-Ambrocio, {Antonio H.} and Ruben Soto-Acosta and Patricia Bautista-Carbajal and Hurtado-Monzon, {Arianna M.} and Alcaraz-Estrada, {Sofia L.} and Ludert, {Juan E.} and {Del Angel}, {Rosa M.}",
year = "2015",
month = "10",
day = "1",
doi = "10.1016/j.virol.2015.05.017",
language = "English (US)",
volume = "484",
pages = "113--126",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Dengue virus NS1 protein interacts with the ribosomal protein RPL18

T2 - This interaction is required for viral translation and replication in Huh-7 cells

AU - Cervantes-Salazar, Margot

AU - Angel-Ambrocio, Antonio H.

AU - Soto-Acosta, Ruben

AU - Bautista-Carbajal, Patricia

AU - Hurtado-Monzon, Arianna M.

AU - Alcaraz-Estrada, Sofia L.

AU - Ludert, Juan E.

AU - Del Angel, Rosa M.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48. hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.

AB - Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48. hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.

KW - Dengue

KW - NS1

KW - Replication

KW - Ribosomal proteins

UR - http://www.scopus.com/inward/record.url?scp=84935893709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84935893709&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2015.05.017

DO - 10.1016/j.virol.2015.05.017

M3 - Article

C2 - 26092250

AN - SCOPUS:84935893709

VL - 484

SP - 113

EP - 126

JO - Virology

JF - Virology

SN - 0042-6822

ER -