TY - JOUR
T1 - Dengue virus NS1 protein interacts with the ribosomal protein RPL18
T2 - This interaction is required for viral translation and replication in Huh-7 cells
AU - Cervantes-Salazar, Margot
AU - Angel-Ambrocio, Antonio H.
AU - Soto-Acosta, Ruben
AU - Bautista-Carbajal, Patricia
AU - Hurtado-Monzon, Arianna M.
AU - Alcaraz-Estrada, Sofia L.
AU - Ludert, Juan E.
AU - Del Angel, Rosa M.
N1 - Funding Information:
The authors wish to thanks to Jaime Zarco and Fernando Medina for their technical assistance to Dr. Juan Salas for the kindly donation of DENV2 strain. This work was supported by CONACYT 127447 , ICyTDF Pifutp8-152 and ICyTDF 248 grants. Margot Cervantes-Salazar, Antonio Angel-Ambrocio, Rubén Soto-Acosta and Patricia Bautista-Carbajal are recipients of CONACYT (Mexico) scholarships.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48. hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.
AB - Given dengue virus (DENV) genome austerity, it uses cellular molecules and structures for virion entry, translation and replication of the genome. NS1 is a multifunctional protein key to viral replication and pathogenesis. Identification of cellular proteins that interact with NS1 may help in further understanding the functions of NS1. In this paper we isolated a total of 64 proteins from DENV infected human hepatic cells (Huh-7) that interact with NS1 by affinity chromatography and immunoprecipitation assays. The subcellular location and expression levels during infection of the ribosomal proteins RPS3a, RPL7, RPL18, RPL18a plus GAPDH were determined. None of these proteins changed their expression levels during infection; however, RPL-18 was redistributed to the perinuclear region after 48. hpi. Silencing of the RPL-18 does not affect cell translation efficiency or viability, but it reduces significantly viral translation, replication and viral yield, suggesting that the RPL-18 is required during DENV replicative cycle.
KW - Dengue
KW - NS1
KW - Replication
KW - Ribosomal proteins
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U2 - 10.1016/j.virol.2015.05.017
DO - 10.1016/j.virol.2015.05.017
M3 - Article
C2 - 26092250
AN - SCOPUS:84935893709
SN - 0042-6822
VL - 484
SP - 113
EP - 126
JO - Virology
JF - Virology
ER -