Dengue virus surveillance: Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil

Tatiana Elias Colombo, Danila Vedovello, Carolina Colombelli Pacca-Mazaro, Adriano Mondini, João Pessoa Araújo, Eliana Cabrera, José Carlos Lopes, Izalco Nuremberg Penha dos Santos, Andréia Francesli Negri Reis, Fabiana Rodrigues Costa, Lilian Elisa Arão Antônio Cruz, Jurandir Ferreira, Eliseu Soares de Oliveira Rocha, Erna Geessien Kroon, Roberta Vieira de Morais Bronzoni, Nikos Vasilakis, Maurício Lacerda Nogueira

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Dengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in São José do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavivirus generic primers, based on the genes of the non-structural protein (NS5), followed by nested-PCR assay with species-specific primers. We analyzed 1549 samples, of which 1389 were positive for NS1 by rapid test. One thousand and eight-seven samples (78%) were confirmed as positive by multiplex RT-PCR: DENV-4, 48.5% (528/1087); DENV-1, 41.5% (449/1087); DENV-2, 9.5% (104/1087); and co-infection (5 DENV-1/DENV-4, 1 DENV-1/DENV-2), 0.5% (6/1087). Phylogenetic analysis of the DENV-4 grouped the isolates identified in this study with the American genotype and the showed a relationship between isolates from SJRP and isolates from the northern region of South America. Taken together, our data shows the detection and emergence of new dengue genotype in a new region and reiterate the importance of surveillance programs to detect and trace the evolution of DENV.

Original languageEnglish (US)
Pages (from-to)84-89
Number of pages6
JournalActa Tropica
Volume164
DOIs
StatePublished - Dec 1 2016

Fingerprint

Dengue virus
Dengue Virus
Brazil
monitoring
Multiplex Polymerase Chain Reaction
Arbovirus Infections
reverse transcriptase polymerase chain reaction
Genotype
Flavivirus
arboviruses
dengue
Dengue
South America
genotype
virus transmission
Coinfection
mixed infection
sampling
health services
fever

Keywords

  • Dengue virus
  • Phylogenetic analysis
  • Surveillance programs
  • Transmission

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Colombo, T. E., Vedovello, D., Pacca-Mazaro, C. C., Mondini, A., Araújo, J. P., Cabrera, E., ... Nogueira, M. L. (2016). Dengue virus surveillance: Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil. Acta Tropica, 164, 84-89. https://doi.org/10.1016/j.actatropica.2016.09.004

Dengue virus surveillance : Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil. / Colombo, Tatiana Elias; Vedovello, Danila; Pacca-Mazaro, Carolina Colombelli; Mondini, Adriano; Araújo, João Pessoa; Cabrera, Eliana; Lopes, José Carlos; Penha dos Santos, Izalco Nuremberg; Negri Reis, Andréia Francesli; Costa, Fabiana Rodrigues; Antônio Cruz, Lilian Elisa Arão; Ferreira, Jurandir; de Oliveira Rocha, Eliseu Soares; Kroon, Erna Geessien; de Morais Bronzoni, Roberta Vieira; Vasilakis, Nikos; Nogueira, Maurício Lacerda.

In: Acta Tropica, Vol. 164, 01.12.2016, p. 84-89.

Research output: Contribution to journalArticle

Colombo, TE, Vedovello, D, Pacca-Mazaro, CC, Mondini, A, Araújo, JP, Cabrera, E, Lopes, JC, Penha dos Santos, IN, Negri Reis, AF, Costa, FR, Antônio Cruz, LEA, Ferreira, J, de Oliveira Rocha, ES, Kroon, EG, de Morais Bronzoni, RV, Vasilakis, N & Nogueira, ML 2016, 'Dengue virus surveillance: Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil', Acta Tropica, vol. 164, pp. 84-89. https://doi.org/10.1016/j.actatropica.2016.09.004
Colombo TE, Vedovello D, Pacca-Mazaro CC, Mondini A, Araújo JP, Cabrera E et al. Dengue virus surveillance: Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil. Acta Tropica. 2016 Dec 1;164:84-89. https://doi.org/10.1016/j.actatropica.2016.09.004
Colombo, Tatiana Elias ; Vedovello, Danila ; Pacca-Mazaro, Carolina Colombelli ; Mondini, Adriano ; Araújo, João Pessoa ; Cabrera, Eliana ; Lopes, José Carlos ; Penha dos Santos, Izalco Nuremberg ; Negri Reis, Andréia Francesli ; Costa, Fabiana Rodrigues ; Antônio Cruz, Lilian Elisa Arão ; Ferreira, Jurandir ; de Oliveira Rocha, Eliseu Soares ; Kroon, Erna Geessien ; de Morais Bronzoni, Roberta Vieira ; Vasilakis, Nikos ; Nogueira, Maurício Lacerda. / Dengue virus surveillance : Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil. In: Acta Tropica. 2016 ; Vol. 164. pp. 84-89.
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