TY - JOUR
T1 - Dengue virus surveillance
T2 - Detection of DENV-4 in the city of São José do Rio Preto, SP, Brazil
AU - Colombo, Tatiana Elias
AU - Vedovello, Danila
AU - Pacca-Mazaro, Carolina Colombelli
AU - Mondini, Adriano
AU - Araújo, João Pessoa
AU - Cabrera, Eliana
AU - Lopes, José Carlos
AU - Penha dos Santos, Izalco Nuremberg
AU - Negri Reis, Andréia Francesli
AU - Costa, Fabiana Rodrigues
AU - Antônio Cruz, Lilian Elisa Arão
AU - Ferreira, Jurandir
AU - de Oliveira Rocha, Eliseu Soares
AU - Kroon, Erna Geessien
AU - de Morais Bronzoni, Roberta Vieira
AU - Vasilakis, Nikos
AU - Nogueira, Maurício Lacerda
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Dengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in São José do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavivirus generic primers, based on the genes of the non-structural protein (NS5), followed by nested-PCR assay with species-specific primers. We analyzed 1549 samples, of which 1389 were positive for NS1 by rapid test. One thousand and eight-seven samples (78%) were confirmed as positive by multiplex RT-PCR: DENV-4, 48.5% (528/1087); DENV-1, 41.5% (449/1087); DENV-2, 9.5% (104/1087); and co-infection (5 DENV-1/DENV-4, 1 DENV-1/DENV-2), 0.5% (6/1087). Phylogenetic analysis of the DENV-4 grouped the isolates identified in this study with the American genotype and the showed a relationship between isolates from SJRP and isolates from the northern region of South America. Taken together, our data shows the detection and emergence of new dengue genotype in a new region and reiterate the importance of surveillance programs to detect and trace the evolution of DENV.
AB - Dengue viruses are the most common arbovirus infection worldwide and are caused by four distinct serotypes of the dengue virus (DENV). In the present study, we assessed DENV transmission in São José do Rio Preto (SJRP) from 2010 to 2014. We analyzed blood samples from febrile patients who were attended at health care centers in SJRP. DENV detection was performed using multiplex RT-PCR, using flavivirus generic primers, based on the genes of the non-structural protein (NS5), followed by nested-PCR assay with species-specific primers. We analyzed 1549 samples, of which 1389 were positive for NS1 by rapid test. One thousand and eight-seven samples (78%) were confirmed as positive by multiplex RT-PCR: DENV-4, 48.5% (528/1087); DENV-1, 41.5% (449/1087); DENV-2, 9.5% (104/1087); and co-infection (5 DENV-1/DENV-4, 1 DENV-1/DENV-2), 0.5% (6/1087). Phylogenetic analysis of the DENV-4 grouped the isolates identified in this study with the American genotype and the showed a relationship between isolates from SJRP and isolates from the northern region of South America. Taken together, our data shows the detection and emergence of new dengue genotype in a new region and reiterate the importance of surveillance programs to detect and trace the evolution of DENV.
KW - Dengue virus
KW - Phylogenetic analysis
KW - Surveillance programs
KW - Transmission
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U2 - 10.1016/j.actatropica.2016.09.004
DO - 10.1016/j.actatropica.2016.09.004
M3 - Article
C2 - 27609639
AN - SCOPUS:84986914873
SN - 0001-706X
VL - 164
SP - 84
EP - 89
JO - Acta Tropica
JF - Acta Tropica
ER -