Deoxyadenosine blockade of G0 to G1 transition in lymphocytes: Possible involvement of protein kinases

Toshiaki Sato, Teh Sheng Chan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Immunodeficiency in adenosine deaminase deficiency has been attributed to the lymphotoxicity of deoxyadenosine that accumulates to high levels in patients. To gain insight into the mechanism of deoxyadenosine toxicity, we investigated the dose-response and time course of its toxic effects on concanavalin A-stimulated mouse splenic lymphocytes by thymidine incorporation and flow cytometry. Deoxyadenosine at a level as low as 0.3 μM inhibited the progression of G0. In contrast, higher concentrations of the nucleoside, i.e., in the range of 1 to 3 μM, were needed to block transition of the stimulated lymphocytes from G0 to G1. The inhibition of their S entry and progression required even higher concentrations. Furthermore, staurosporine, a potent inhibitor of protein kinases, was found to potentiate the toxicity of deoxyadenosine in mitogen-stimulated lymphocytes. Calcium mobilization in mitogen-activated lymphocytes was inhibited by deoxyadenosine. Our data suggest that, while ribonucleotide reductase inhibition by dATP could explain the blockade of S entry and progression by deoxyadenosine in cycling lymphocytes or leukemic cells, more important effects of this compound on antigen-activated lymphocytes occur at the early G0 phase. A possible mechanism of deoxyadenosine lethality is its inhibition of protein phosphorylation.

Original languageEnglish (US)
Pages (from-to)288-295
Number of pages8
JournalJournal of Cellular Physiology
Volume166
Issue number2
DOIs
StatePublished - Feb 1996

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Lymphocytes
Protein Kinases
Mitogens
Toxicity
Ribonucleotide Reductases
Cell Cycle Resting Phase
Phosphorylation
Adenosine Deaminase
Staurosporine
Flow cytometry
Poisons
Protein Kinase Inhibitors
Concanavalin A
2'-deoxyadenosine
Nucleosides
Thymidine
Reaction Time
Flow Cytometry
Calcium
Antigens

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Deoxyadenosine blockade of G0 to G1 transition in lymphocytes : Possible involvement of protein kinases. / Sato, Toshiaki; Chan, Teh Sheng.

In: Journal of Cellular Physiology, Vol. 166, No. 2, 02.1996, p. 288-295.

Research output: Contribution to journalArticle

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