Deoxynucleotide triphosphate binding mode conserved in Y family DNA polymerases

Robert E. Johnson, José Trincao, Aneel K. Aggarwal, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Although DNA polymerase η (Polη) and other Y family polymerases differ in sequence and function from classical DNA polymerases, they all share a similar right-handed architecture with the palm, fingers, and thumb domains. Here, we examine the role in Saccharomyces cerevisiae Polη of three conserved residues, tyrosine 64, arginine 67, and lysine 279, which come into close contact with the triphosphate moiety of the incoming nucleotide, in nucleotide incorporation. We find that mutational alteration of these residues reduces the efficiency of correct nucleotide incorporation very considerably. The high degree of conservation of these residues among the various Y family DNA polymerases suggests that these residues are also crucial for nucleotide incorporation in the other members of the family. Furthermore, we note that tyrosine 64 and arginine 67 are functionally equivalent to the deoxynucleotide triphosphate binding residues arginine 518 and histidine 506 in T7 DNA polymerase, respectively.

Original languageEnglish
Pages (from-to)3008-3012
Number of pages5
JournalMolecular and Cellular Biology
Volume23
Issue number8
DOIs
StatePublished - Apr 2003

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DNA-Directed DNA Polymerase
Nucleotides
Arginine
Tyrosine
Thumb
Histidine
Fingers
Lysine
Saccharomyces cerevisiae
triphosphoric acid

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Deoxynucleotide triphosphate binding mode conserved in Y family DNA polymerases. / Johnson, Robert E.; Trincao, José; Aggarwal, Aneel K.; Prakash, Satya; Prakash, Louise.

In: Molecular and Cellular Biology, Vol. 23, No. 8, 04.2003, p. 3008-3012.

Research output: Contribution to journalArticle

Johnson, Robert E. ; Trincao, José ; Aggarwal, Aneel K. ; Prakash, Satya ; Prakash, Louise. / Deoxynucleotide triphosphate binding mode conserved in Y family DNA polymerases. In: Molecular and Cellular Biology. 2003 ; Vol. 23, No. 8. pp. 3008-3012.
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