Description of an endometrioid ovarian cancer cell line

Francesca R. Giancotti, Brent H. Dorsett, Scott C. Weaver, Rajendra Bharathur, Harry L. Ioachim, Hugh R.K. Barber

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A human cell line, designated L-1, has been established from the ascites of an untreated patient with stage IV (FIGO) endometrioid ovarian cancer. This cell line initially grew uninterrupted for 6 months without fibroblast contamination and contact inhibition, and has been subcultured weekly for the past 7 years. L-1 does not contain steroid hormone receptors nor does it demonstrate the presence of oncofetal antigens by immunohistochemical techniques. The doubling time of L-1 is 11.8 hr. Flow cytometric analysis reveals an aneuploid DNA peak, and an abnormal karyotype demonstrates hyperdiploidy, translocations, and deletions. Morphology, growth patterns, cytogenetic analysis, and other features of L-1 are characterized.

Original languageEnglish (US)
Pages (from-to)330-337
Number of pages8
JournalGynecologic Oncology
Volume35
Issue number3
DOIs
StatePublished - Dec 1989
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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    Giancotti, F. R., Dorsett, B. H., Weaver, S. C., Bharathur, R., Ioachim, H. L., & Barber, H. R. K. (1989). Description of an endometrioid ovarian cancer cell line. Gynecologic Oncology, 35(3), 330-337. https://doi.org/10.1016/0090-8258(89)90073-5