Description of an endometrioid ovarian cancer cell line

Francesca R. Giancotti, Brent H. Dorsett, Scott Weaver, Rajendra Bharathur, Harry L. Ioachim, Hugh R K Barber

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A human cell line, designated L-1, has been established from the ascites of an untreated patient with stage IV (FIGO) endometrioid ovarian cancer. This cell line initially grew uninterrupted for 6 months without fibroblast contamination and contact inhibition, and has been subcultured weekly for the past 7 years. L-1 does not contain steroid hormone receptors nor does it demonstrate the presence of oncofetal antigens by immunohistochemical techniques. The doubling time of L-1 is 11.8 hr. Flow cytometric analysis reveals an aneuploid DNA peak, and an abnormal karyotype demonstrates hyperdiploidy, translocations, and deletions. Morphology, growth patterns, cytogenetic analysis, and other features of L-1 are characterized.

Original languageEnglish
Pages (from-to)330-337
Number of pages8
JournalGynecologic Oncology
Volume35
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes

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Ovarian Neoplasms
Contact Inhibition
Abnormal Karyotype
Cell Line
Polyploidy
Steroid Receptors
Cytogenetic Analysis
Aneuploidy
Ascites
Fibroblasts
Hormones
DNA
Growth
oncofetal antigens

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Giancotti, F. R., Dorsett, B. H., Weaver, S., Bharathur, R., Ioachim, H. L., & Barber, H. R. K. (1989). Description of an endometrioid ovarian cancer cell line. Gynecologic Oncology, 35(3), 330-337. https://doi.org/10.1016/0090-8258(89)90073-5

Description of an endometrioid ovarian cancer cell line. / Giancotti, Francesca R.; Dorsett, Brent H.; Weaver, Scott; Bharathur, Rajendra; Ioachim, Harry L.; Barber, Hugh R K.

In: Gynecologic Oncology, Vol. 35, No. 3, 1989, p. 330-337.

Research output: Contribution to journalArticle

Giancotti, FR, Dorsett, BH, Weaver, S, Bharathur, R, Ioachim, HL & Barber, HRK 1989, 'Description of an endometrioid ovarian cancer cell line', Gynecologic Oncology, vol. 35, no. 3, pp. 330-337. https://doi.org/10.1016/0090-8258(89)90073-5
Giancotti FR, Dorsett BH, Weaver S, Bharathur R, Ioachim HL, Barber HRK. Description of an endometrioid ovarian cancer cell line. Gynecologic Oncology. 1989;35(3):330-337. https://doi.org/10.1016/0090-8258(89)90073-5
Giancotti, Francesca R. ; Dorsett, Brent H. ; Weaver, Scott ; Bharathur, Rajendra ; Ioachim, Harry L. ; Barber, Hugh R K. / Description of an endometrioid ovarian cancer cell line. In: Gynecologic Oncology. 1989 ; Vol. 35, No. 3. pp. 330-337.
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