Desensitization of β-adrenergic receptors in adipocytes causes increased insulin sensitivity of glucose transport

Allan Green, Richard Carroll, Susan B. Dobias

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21 Citations (Scopus)

Abstract

To determine the effect of desensitization of adipocyte β-adrenergic receptors on insulin sensitivity, rats were continuously infused with isoproterenol (50 or 100 μg · kg-1 · h-1) for 3 days by osmotic minipumps. Epididymal adipocytes were isolated. The cells from treated animals were desensitized to isoproterenol, as determined by response of lipolysis (glycerol release). Binding of [125I]iodocyanopindolol was decreased by ~80% in adipocyte plasma membranes isolated from treated rats, indicating that β-adrenergic receptors were downregulated. Cellular concentrations of G(s)α and G(i)α were not altered. Insulin sensitivity was determined by measuring the effect of insulin on glucose transport (2- deoxy[3H]glucose uptake). Cells from the isoproterenol-infused rats were markedly more sensitive to insulin than those from control rats. This was evidenced by an ~50% increase in maximal glucose transport rate in cells from the high-dose isoproterenol-treated rats and by an ~40% decrease in the half-maximal effective concentration of insulin in both groups. 125I- labeled insulin binding to adipocytes was not altered by the isoproterenol infusions, indicating that desensitization of β-adrenergic receptors results in tighter coupling between insulin receptors and stimulation of glucose transport.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume271
Issue number2 34-2
StatePublished - Aug 1996

Fingerprint

Isoproterenol
Adipocytes
Adrenergic Receptors
Insulin Resistance
Insulin
Glucose
Rats
Iodocyanopindolol
Rat control
Lipolysis
Insulin Receptor
Glycerol
Cell membranes
Down-Regulation
Cell Membrane
Animals

Keywords

  • catecholamines
  • insulin

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

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abstract = "To determine the effect of desensitization of adipocyte β-adrenergic receptors on insulin sensitivity, rats were continuously infused with isoproterenol (50 or 100 μg · kg-1 · h-1) for 3 days by osmotic minipumps. Epididymal adipocytes were isolated. The cells from treated animals were desensitized to isoproterenol, as determined by response of lipolysis (glycerol release). Binding of [125I]iodocyanopindolol was decreased by ~80{\%} in adipocyte plasma membranes isolated from treated rats, indicating that β-adrenergic receptors were downregulated. Cellular concentrations of G(s)α and G(i)α were not altered. Insulin sensitivity was determined by measuring the effect of insulin on glucose transport (2- deoxy[3H]glucose uptake). Cells from the isoproterenol-infused rats were markedly more sensitive to insulin than those from control rats. This was evidenced by an ~50{\%} increase in maximal glucose transport rate in cells from the high-dose isoproterenol-treated rats and by an ~40{\%} decrease in the half-maximal effective concentration of insulin in both groups. 125I- labeled insulin binding to adipocytes was not altered by the isoproterenol infusions, indicating that desensitization of β-adrenergic receptors results in tighter coupling between insulin receptors and stimulation of glucose transport.",
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