TY - JOUR
T1 - Design of SERS nanotags for multiplexed lateral flow immunoassays
AU - Sánchez-Purrà, Maria
AU - Roig-Solvas, Biel
AU - Versiani, Alice
AU - Rodriguez-Quijada, Cristina
AU - De Puig, Helena
AU - Bosch, Irene
AU - Gehrke, Lee
AU - Hamad-Schifferli, Kimberly
N1 - Funding Information:
We thank the UMB Engineering Department for use of the Raman microscope. Work was supported by funding from the NIH NIAID (AI100190). We would like to thank Dr. Tom Tague and Dr. Peng Wang from Bruker Optics for technical assistance, and Dr. Justina Tam from the US FDA.
Funding Information:
HdP was supported by the MIT/SUTD International Design Centre, a Broshy Fellowship and a MIT Tata Center for Technology and Design Fellowship. Both HdP and CRQ were supported by Rafael del Pino Fellowships.
Publisher Copyright:
© The Royal Society of Chemistry 2018.
PY - 2017
Y1 - 2017
N2 - Surface enhanced Raman spectroscopy (SERS) has been attractive for enhancing the sensitivity of lateral flow immunoassays (LFA). A format that has enabled specific detection of biomarkers is to use Raman reporter molecules linked to gold nanoparticles (NPs), which are conjugated to laboratory made antibodies specific for the target of interest. Many factors such as the NP and Ab properties and the method of signal readout impact the sensitivity of a SERS based immunoassay. To understand how to optimize assay sensitivity, we studied SERS readouts of multiplexed sandwich immunoassays for the zika and dengue non-structural protein 1 (NS1) biomarkers as a test case. We investigated the effect of NP shape on the SERS enhancement of the reporter molecules 1,2-bis(4-pyridyl)ethylene (BPE) and 4-mercaptobenzoic acid (MBA). We also performed SERS imaging of test lines to map the spatial distribution of signal in test lines on the nitrocellulose. Finally, we used a modified least squares analysis to differentiate reporter contributions.
AB - Surface enhanced Raman spectroscopy (SERS) has been attractive for enhancing the sensitivity of lateral flow immunoassays (LFA). A format that has enabled specific detection of biomarkers is to use Raman reporter molecules linked to gold nanoparticles (NPs), which are conjugated to laboratory made antibodies specific for the target of interest. Many factors such as the NP and Ab properties and the method of signal readout impact the sensitivity of a SERS based immunoassay. To understand how to optimize assay sensitivity, we studied SERS readouts of multiplexed sandwich immunoassays for the zika and dengue non-structural protein 1 (NS1) biomarkers as a test case. We investigated the effect of NP shape on the SERS enhancement of the reporter molecules 1,2-bis(4-pyridyl)ethylene (BPE) and 4-mercaptobenzoic acid (MBA). We also performed SERS imaging of test lines to map the spatial distribution of signal in test lines on the nitrocellulose. Finally, we used a modified least squares analysis to differentiate reporter contributions.
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U2 - 10.1039/c7me00052a
DO - 10.1039/c7me00052a
M3 - Article
AN - SCOPUS:85042322781
SN - 2058-9689
VL - 2
SP - 401
EP - 409
JO - Molecular Systems Design and Engineering
JF - Molecular Systems Design and Engineering
IS - 4
ER -