Design of SERS nanotags for multiplexed lateral flow immunoassays

Maria Sánchez-Purrà, Biel Roig-Solvas, Alice Versiani, Cristina Rodriguez-Quijada, Helena De Puig, Irene Bosch, Lee Gehrke, Kimberly Hamad-Schifferli

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Surface enhanced Raman spectroscopy (SERS) has been attractive for enhancing the sensitivity of lateral flow immunoassays (LFA). A format that has enabled specific detection of biomarkers is to use Raman reporter molecules linked to gold nanoparticles (NPs), which are conjugated to laboratory made antibodies specific for the target of interest. Many factors such as the NP and Ab properties and the method of signal readout impact the sensitivity of a SERS based immunoassay. To understand how to optimize assay sensitivity, we studied SERS readouts of multiplexed sandwich immunoassays for the zika and dengue non-structural protein 1 (NS1) biomarkers as a test case. We investigated the effect of NP shape on the SERS enhancement of the reporter molecules 1,2-bis(4-pyridyl)ethylene (BPE) and 4-mercaptobenzoic acid (MBA). We also performed SERS imaging of test lines to map the spatial distribution of signal in test lines on the nitrocellulose. Finally, we used a modified least squares analysis to differentiate reporter contributions.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalMolecular Systems Design and Engineering
Issue number4
StatePublished - 2017
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Chemical Engineering (miscellaneous)
  • Biomedical Engineering
  • Energy Engineering and Power Technology
  • Process Chemistry and Technology
  • Industrial and Manufacturing Engineering
  • Materials Chemistry


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