Detecting Counterion Dynamics in DNA–Protein Association

Channing C. Pletka, Ridvan Nepravishta, Junji Iwahara

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Due to a high density of negative charges on its surface, DNA condenses cations as counterions, forming the so-called “ion atmosphere”. Although the release of counterions upon DNA–protein association has been postulated to have a major contribution to the binding thermodynamics, this release remains to be confirmed through a direct observation of the ions. Herein, we report the characterization of the ion atmosphere around DNA using NMR spectroscopy and directly detect the release of counterions upon DNA–protein association. NMR-based diffusion data reveal the highly dynamic nature of counterions within the ion atmosphere around DNA. Counterion release is observed as an increase in the apparent ionic diffusion coefficient, which directly provides the number of counterions released upon DNA–protein association.

Original languageEnglish (US)
Pages (from-to)1465-1468
Number of pages4
JournalAngewandte Chemie - International Edition
Volume59
Issue number4
DOIs
StatePublished - Jan 20 2020

Keywords

  • DNA recognition
  • NMR spectroscopy
  • dynamics
  • ion pairs
  • protein–DNA interactions

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Fingerprint Dive into the research topics of 'Detecting Counterion Dynamics in DNA–Protein Association'. Together they form a unique fingerprint.

  • Cite this