Detecting potential IgE-reactive sites on food proteins using a sequence and structure database, SDAP-food

Ovidiu Ivanciuc, Venkatarajan Mathura, Terumi Midoro-Horiuti, Werner Braun, Randall M. Goldblum, Catherine H. Schein

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The high incidence of food allergies, including oral allergy syndrome, represent major considerations when introducing new crops and foods. A new structural database of allergenic proteins, SDAP-Food, http://fermi.utmb.edu/SDAP/, has been developed to aid in predicting the IgE-binding potential of novel food proteins and cross-reactivities among known allergens. The site is designed to facilitate the first steps of a decision tree approach to determine the allergenicity of a given protein, based on the sequence and structural similarity to known allergens and their IgE binding sites. Immunological tests can then be used to confirm the predictions. A hierarchical procedure for identifying potential allergens, using a physical property-based sequence similarity index, has been designed to identify regions that resemble known IgE binding sites. As an example, SDAP tools were used to find food allergen sequences similar to an IgE binding site of the Jun a 3 allergen from mountain cedar pollen. The SDAP sequence similarity search matched the Jun a 3 epitope to regions in several food allergens, including cherry (Pru av 2), apple (Mal d 2) and pepper (Cap a 1), which are, like Jun a 3, members of the plant pathogenesis-related (PR-5) protein family. Homology modeling, using our EXDIS/DIAMOD/FANTOM program suite, indicated a similar surface location and structure for the potential epitope region on all of these allergens. The quantitative approach presented here can be used as part of a screening process for potential allergenicity of recombinant food products.

Original languageEnglish (US)
Pages (from-to)4830-4837
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number16
DOIs
StatePublished - Jul 30 2003

Fingerprint

protein structure
allergens
active sites
Allergens
Immunoglobulin E
Catalytic Domain
amino acid sequences
Databases
Food
Proteins
Allergies
binding sites
allergenicity
Binding Sites
epitopes
Epitopes
Decision Trees
Protein Databases
Immunologic Tests
novel foods

Keywords

  • Allergen structure
  • Cap a 1
  • Jun a 3
  • Mal d 2
  • Oral allergy syndrome
  • Pathogenesis related plant proteins
  • PR-5
  • Pru av 2

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Detecting potential IgE-reactive sites on food proteins using a sequence and structure database, SDAP-food. / Ivanciuc, Ovidiu; Mathura, Venkatarajan; Midoro-Horiuti, Terumi; Braun, Werner; Goldblum, Randall M.; Schein, Catherine H.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 16, 30.07.2003, p. 4830-4837.

Research output: Contribution to journalArticle

Ivanciuc, Ovidiu ; Mathura, Venkatarajan ; Midoro-Horiuti, Terumi ; Braun, Werner ; Goldblum, Randall M. ; Schein, Catherine H. / Detecting potential IgE-reactive sites on food proteins using a sequence and structure database, SDAP-food. In: Journal of Agricultural and Food Chemistry. 2003 ; Vol. 51, No. 16. pp. 4830-4837.
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