Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells

Y. Deo, H. Ghebremariam, M. Cloyd

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Ecotropic recombinant virus (ERV), a relatively new class of murine retrovirus endogenous to mice, is expressed at significant levels by most murine myeloma and hybridoma cells examined. The routine XC, S+L-, mink cell focus-inducing (MCF), and reverse transcriptase (RT) tests are not suitable to detect and quantify the levels of ERV. A serological focus assay, based on specific anti-murine leukemia virus (MuLV) viral envelope (env) antibodies, is required to detect ERV. A more sensitive format of this serological focus assay includes co-cultivation of test article cells with the indicator (Mus dunni) cells. ERV isolated from murine hybridoma cells show a unique pattern of cross-reactivity with anti-MuLV env antibodies and this pattern is clearly distinct from that of ectropic and xenotropic retroviruses.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalHybridoma
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology
  • Genetics

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