Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells

Y. Deo, H. Ghebremariam, M. Cloyd

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Ecotropic recombinant virus (ERV), a relatively new class of murine retrovirus endogenous to mice, is expressed at significant levels by most murine myeloma and hybridoma cells examined. The routine XC, S+L-, mink cell focus-inducing (MCF), and reverse transcriptase (RT) tests are not suitable to detect and quantify the levels of ERV. A serological focus assay, based on specific anti-murine leukemia virus (MuLV) viral envelope (env) antibodies, is required to detect ERV. A more sensitive format of this serological focus assay includes co-cultivation of test article cells with the indicator (Mus dunni) cells. ERV isolated from murine hybridoma cells show a unique pattern of cross-reactivity with anti-MuLV env antibodies and this pattern is clearly distinct from that of ectropic and xenotropic retroviruses.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalHybridoma
Volume13
Issue number1
StatePublished - 1994

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Hybridomas
Viruses
Murine Leukemia Viruses
Viral Antibodies
Endogenous Retroviruses
Mink
RNA-Directed DNA Polymerase
Retroviridae
Antibodies

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells. / Deo, Y.; Ghebremariam, H.; Cloyd, M.

In: Hybridoma, Vol. 13, No. 1, 1994, p. 69-76.

Research output: Contribution to journalArticle

Deo, Y, Ghebremariam, H & Cloyd, M 1994, 'Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells', Hybridoma, vol. 13, no. 1, pp. 69-76.
Deo, Y. ; Ghebremariam, H. ; Cloyd, M. / Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells. In: Hybridoma. 1994 ; Vol. 13, No. 1. pp. 69-76.
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