Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates

Anthony Sanchez, Thomas Ksiazek, Pierre E. Rollin, Mary E G Miranda, Sam G. Trappier, Ali S. Khan, Clarence J. Peters, Stuart T. Nichol

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Ebola (EBO) viruses were detected in specimens obtained during the hemorrhagic fever outbreak among humans in Kikwit, Democratic Republic of the Congo (DRC), in 1995 (subtype Zaire) and during an outbreak of disease in cynomolgus macaques in Alice, Texas, and the Philippines in 1996 (subtype Reston). Reverse transcriptase-polymerase chain reaction assays were developed and proven effective for detecting vital RNA in body fluids and tissues of infected individuals. Little change was seen in the nucleotide or deduced amino acid sequences of the glycoprotein (GP) of these EBO virus subtypes compared with those of their original representatives (i.e., the 1976 Yambuku, DRC, EBO isolate [subtype Zaire] and the 1989 Philippines and Reston, Virginia, isolates [subtype Reston]). The nonstructural secreted GP (SGP), the primary product of the GP gene, was more highly conserved than the structural GP, indicating different functional roles or evolutionary constraints for these proteins. Significant amounts of SGP were detected in acutely infected humans.

Original languageEnglish (US)
JournalJournal of Infectious Diseases
Volume179
Issue numberSUPPL. 1
StatePublished - 1999
Externally publishedYes

Fingerprint

Ebola Hemorrhagic Fever
Democratic Republic of the Congo
Primates
Ebolavirus
Glycoproteins
Philippines
Disease Outbreaks
Macaca
Body Fluids
Reverse Transcriptase Polymerase Chain Reaction
Amino Acid Sequence
Fever
Nucleotides
RNA
Genes
Reston
Proteins

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Sanchez, A., Ksiazek, T., Rollin, P. E., Miranda, M. E. G., Trappier, S. G., Khan, A. S., ... Nichol, S. T. (1999). Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates. Journal of Infectious Diseases, 179(SUPPL. 1).

Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates. / Sanchez, Anthony; Ksiazek, Thomas; Rollin, Pierre E.; Miranda, Mary E G; Trappier, Sam G.; Khan, Ali S.; Peters, Clarence J.; Nichol, Stuart T.

In: Journal of Infectious Diseases, Vol. 179, No. SUPPL. 1, 1999.

Research output: Contribution to journalArticle

Sanchez, A, Ksiazek, T, Rollin, PE, Miranda, MEG, Trappier, SG, Khan, AS, Peters, CJ & Nichol, ST 1999, 'Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates', Journal of Infectious Diseases, vol. 179, no. SUPPL. 1.
Sanchez, Anthony ; Ksiazek, Thomas ; Rollin, Pierre E. ; Miranda, Mary E G ; Trappier, Sam G. ; Khan, Ali S. ; Peters, Clarence J. ; Nichol, Stuart T. / Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates. In: Journal of Infectious Diseases. 1999 ; Vol. 179, No. SUPPL. 1.
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