Detection and phylogenetic analysis of Orf virus from sheep in Brazil: A case report

Jônatas S. Abrahão; Rafael K. Campos; Giliane S. Trindade; Maria Im Guedes; Zélia Ip Lobato; Carlos Mazur; Paulo Cp Ferreira; Cláudio A. Bonjardim; Erna G. Kroon

doi:10.1186/1743-422X-6-47

Detection and phylogenetic analysis of Orf virus from sheep in Brazil: A case report

Jônatas S. Abrahão, Rafael K. Campos, Giliane S. Trindade, Maria Im Guedes, Zélia Ip Lobato, Carlos Mazur, Paulo Cp Ferreira, Cláudio A. Bonjardim, Erna G. Kroon

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45 Scopus citations

Abstract

Background: Orf virus (ORFV), the prototype of the genus Parapoxvirus (PPV), is the etiological agent of contagious ecthyma, a severe exanthematic dermatitis that afflicts domestic and wild small ruminants. Although South American ORFV outbreaks have occurred and diagnosed there are no South American PPV major membrane glycoprotein B2L gene nucleotide sequences available. Case presentation: an outbreak of ovine contagious ecthyma in Midwest Brazil was investigated. The diagnosis was based on clinical examinations and molecular biology techniques. The molecular characterization of the virus was done using PCR amplification, cloning and DNA sequencing of the B2L gene. The phylogenetic analysis demonstrated a high degree of identity with ORFV strains, and the isolate was closest to the ORFV-India 82/04 isolate. Another Brazilian ORFV isolate, NE1, was sequenced for comparative analysis and also showed a high degree of identity with an Asian ORFV strain. Conclusion: Distinct ORFV strains are circulating in Brazil. This is the first report on the phylogenetic analysis of an ORFV in South America.

Original language	English (US)
Article number	47
Journal	Virology journal
Volume	6
DOIs	https://doi.org/10.1186/1743-422X-6-47
State	Published - 2009
Externally published	Yes

ASJC Scopus subject areas

Virology
Infectious Diseases

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@article{345bdff831dc43bb9aadc7dfe4f878de,

title = "Detection and phylogenetic analysis of Orf virus from sheep in Brazil: A case report",

abstract = "Background: Orf virus (ORFV), the prototype of the genus Parapoxvirus (PPV), is the etiological agent of contagious ecthyma, a severe exanthematic dermatitis that afflicts domestic and wild small ruminants. Although South American ORFV outbreaks have occurred and diagnosed there are no South American PPV major membrane glycoprotein B2L gene nucleotide sequences available. Case presentation: an outbreak of ovine contagious ecthyma in Midwest Brazil was investigated. The diagnosis was based on clinical examinations and molecular biology techniques. The molecular characterization of the virus was done using PCR amplification, cloning and DNA sequencing of the B2L gene. The phylogenetic analysis demonstrated a high degree of identity with ORFV strains, and the isolate was closest to the ORFV-India 82/04 isolate. Another Brazilian ORFV isolate, NE1, was sequenced for comparative analysis and also showed a high degree of identity with an Asian ORFV strain. Conclusion: Distinct ORFV strains are circulating in Brazil. This is the first report on the phylogenetic analysis of an ORFV in South America.",

author = "Abrah{\~a}o, {J{\^o}natas S.} and Campos, {Rafael K.} and Trindade, {Giliane S.} and Guedes, {Maria Im} and Lobato, {Z{\'e}lia Ip} and Carlos Mazur and Ferreira, {Paulo Cp} and Bonjardim, {Cl{\'a}udio A.} and Kroon, {Erna G.}",

note = "Funding Information: We thank MSc. Jo{\~a}o R. dos Santos, Angela S. Lopes, Ilda M.V. Gamma, and colleagues from Laborat{\'o}rio de V{\'i}rus (ICB-UFMG). Financial support was provided by CNPq, CAPES and FAPEMIG. EGK, CAB and PCPF received fellowship from CNPq.",

year = "2009",

doi = "10.1186/1743-422X-6-47",

language = "English (US)",

volume = "6",

journal = "Virology journal",

issn = "1743-422X",

publisher = "BioMed Central",

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TY - JOUR

T1 - Detection and phylogenetic analysis of Orf virus from sheep in Brazil

T2 - A case report

AU - Abrahão, Jônatas S.

AU - Campos, Rafael K.

AU - Trindade, Giliane S.

AU - Guedes, Maria Im

AU - Lobato, Zélia Ip

AU - Mazur, Carlos

AU - Ferreira, Paulo Cp

AU - Bonjardim, Cláudio A.

AU - Kroon, Erna G.

N1 - Funding Information: We thank MSc. João R. dos Santos, Angela S. Lopes, Ilda M.V. Gamma, and colleagues from Laboratório de Vírus (ICB-UFMG). Financial support was provided by CNPq, CAPES and FAPEMIG. EGK, CAB and PCPF received fellowship from CNPq.

PY - 2009

Y1 - 2009

N2 - Background: Orf virus (ORFV), the prototype of the genus Parapoxvirus (PPV), is the etiological agent of contagious ecthyma, a severe exanthematic dermatitis that afflicts domestic and wild small ruminants. Although South American ORFV outbreaks have occurred and diagnosed there are no South American PPV major membrane glycoprotein B2L gene nucleotide sequences available. Case presentation: an outbreak of ovine contagious ecthyma in Midwest Brazil was investigated. The diagnosis was based on clinical examinations and molecular biology techniques. The molecular characterization of the virus was done using PCR amplification, cloning and DNA sequencing of the B2L gene. The phylogenetic analysis demonstrated a high degree of identity with ORFV strains, and the isolate was closest to the ORFV-India 82/04 isolate. Another Brazilian ORFV isolate, NE1, was sequenced for comparative analysis and also showed a high degree of identity with an Asian ORFV strain. Conclusion: Distinct ORFV strains are circulating in Brazil. This is the first report on the phylogenetic analysis of an ORFV in South America.

AB - Background: Orf virus (ORFV), the prototype of the genus Parapoxvirus (PPV), is the etiological agent of contagious ecthyma, a severe exanthematic dermatitis that afflicts domestic and wild small ruminants. Although South American ORFV outbreaks have occurred and diagnosed there are no South American PPV major membrane glycoprotein B2L gene nucleotide sequences available. Case presentation: an outbreak of ovine contagious ecthyma in Midwest Brazil was investigated. The diagnosis was based on clinical examinations and molecular biology techniques. The molecular characterization of the virus was done using PCR amplification, cloning and DNA sequencing of the B2L gene. The phylogenetic analysis demonstrated a high degree of identity with ORFV strains, and the isolate was closest to the ORFV-India 82/04 isolate. Another Brazilian ORFV isolate, NE1, was sequenced for comparative analysis and also showed a high degree of identity with an Asian ORFV strain. Conclusion: Distinct ORFV strains are circulating in Brazil. This is the first report on the phylogenetic analysis of an ORFV in South America.

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Detection and phylogenetic analysis of Orf virus from sheep in Brazil: A case report

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