Detection and ultrastructural localization of human smooth muscle myosin like molecules in human non muscle cells by specific antibodies

R. G. Painter, Michael Sheetz, S. J. Singer

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Spectrin, a protein complex which is peripherally attached to the cytoplasmic surface of the human erythrocyte membrane, cannot be detected (by complement fixation with anti spectrin antibodies) in homogenates of several different human non muscle cells studied. On the other hand, a protein antigenically identical or similar to human smooth muscle myosin was detected (by complement fixation with antibodies to uterine smooth muscle myosin) in these cells. In the case of human fibroblast line WI38, this smooth muscle myosin like component was shown (by ferritin antibody experiments in electron microscopy) to be at least partly associated with the cytoplasmic surface of the plasma membrane of the cell. It is proposed that the spectrin complex of the erythrocyte membrane and the smooth muscle myosin like component of the fibroblast membrane play similar roles in regulating the translational mobilities of integral proteins in their respective membranes.

Original languageEnglish (US)
Pages (from-to)1359-1363
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number4
DOIs
StatePublished - Jan 1 1975
Externally publishedYes

Fingerprint

Smooth Muscle Myosins
Spectrin
Muscle Cells
Antibodies
Erythrocyte Membrane
Fibroblasts
Proteins
Membranes
Myometrium
Ferritins
Plasma Cells
Anti-Idiotypic Antibodies
Electron Microscopy
Cell Membrane

ASJC Scopus subject areas

  • General

Cite this

@article{9aa902cf0c23432e9b1261da3602498f,
title = "Detection and ultrastructural localization of human smooth muscle myosin like molecules in human non muscle cells by specific antibodies",
abstract = "Spectrin, a protein complex which is peripherally attached to the cytoplasmic surface of the human erythrocyte membrane, cannot be detected (by complement fixation with anti spectrin antibodies) in homogenates of several different human non muscle cells studied. On the other hand, a protein antigenically identical or similar to human smooth muscle myosin was detected (by complement fixation with antibodies to uterine smooth muscle myosin) in these cells. In the case of human fibroblast line WI38, this smooth muscle myosin like component was shown (by ferritin antibody experiments in electron microscopy) to be at least partly associated with the cytoplasmic surface of the plasma membrane of the cell. It is proposed that the spectrin complex of the erythrocyte membrane and the smooth muscle myosin like component of the fibroblast membrane play similar roles in regulating the translational mobilities of integral proteins in their respective membranes.",
author = "Painter, {R. G.} and Michael Sheetz and Singer, {S. J.}",
year = "1975",
month = "1",
day = "1",
doi = "10.1073/pnas.72.4.1359",
language = "English (US)",
volume = "72",
pages = "1359--1363",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "4",

}

TY - JOUR

T1 - Detection and ultrastructural localization of human smooth muscle myosin like molecules in human non muscle cells by specific antibodies

AU - Painter, R. G.

AU - Sheetz, Michael

AU - Singer, S. J.

PY - 1975/1/1

Y1 - 1975/1/1

N2 - Spectrin, a protein complex which is peripherally attached to the cytoplasmic surface of the human erythrocyte membrane, cannot be detected (by complement fixation with anti spectrin antibodies) in homogenates of several different human non muscle cells studied. On the other hand, a protein antigenically identical or similar to human smooth muscle myosin was detected (by complement fixation with antibodies to uterine smooth muscle myosin) in these cells. In the case of human fibroblast line WI38, this smooth muscle myosin like component was shown (by ferritin antibody experiments in electron microscopy) to be at least partly associated with the cytoplasmic surface of the plasma membrane of the cell. It is proposed that the spectrin complex of the erythrocyte membrane and the smooth muscle myosin like component of the fibroblast membrane play similar roles in regulating the translational mobilities of integral proteins in their respective membranes.

AB - Spectrin, a protein complex which is peripherally attached to the cytoplasmic surface of the human erythrocyte membrane, cannot be detected (by complement fixation with anti spectrin antibodies) in homogenates of several different human non muscle cells studied. On the other hand, a protein antigenically identical or similar to human smooth muscle myosin was detected (by complement fixation with antibodies to uterine smooth muscle myosin) in these cells. In the case of human fibroblast line WI38, this smooth muscle myosin like component was shown (by ferritin antibody experiments in electron microscopy) to be at least partly associated with the cytoplasmic surface of the plasma membrane of the cell. It is proposed that the spectrin complex of the erythrocyte membrane and the smooth muscle myosin like component of the fibroblast membrane play similar roles in regulating the translational mobilities of integral proteins in their respective membranes.

UR - http://www.scopus.com/inward/record.url?scp=0016803940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016803940&partnerID=8YFLogxK

U2 - 10.1073/pnas.72.4.1359

DO - 10.1073/pnas.72.4.1359

M3 - Article

VL - 72

SP - 1359

EP - 1363

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 4

ER -