Detection of 8-hydroxy-2'-deoxyguanosine In Deoxyribonucleic Acid By The 32p-postlabeling Method

Lee jane W. Lu, Kohfuku Kohda, Fumiyo Tasaka, James A. Hokanson

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Using synthesized 8-hydroxy-2'-deoxyguanosine 3'-monophosphate as a marker, the 32P-postlabeling method was adapted with minimum modifications for the analysis of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content in deoxyribonucleic acid (DNA). This method allows the analysis of one 8-OH-dG per 104 DNA nucleotides with only 10 pmoles of nucleotides required. The amounts of 8-OH-dG in DNA detected by the postlabeling method correlated well with the electrochemical detection method but were consistently lower.

Original languageEnglish (US)
Pages (from-to)1880-1882
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume39
Issue number7
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Keywords

  • 32P-postlabeling
  • 8-hydroxydeoxyguanosine
  • DNA
  • oxidative damage

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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