Detection of 8-hydroxy-2'-deoxyguanosine in deoxyribonucleic acid by the 32P-postlabeling method

Leejane Lu, F. Tasaka, J. A. Hokanson, K. Kohda

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Using synthesized 8-hydroxy-2'-deoxyguanosine 3'-monophosphate as a marker, the 32P-postlabeling method was adapted with minimum modifications for the analysis of 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content in deoxyribonucleic acid (DNA). This method allows the analysis of one 8-OH-dG per 104 DNA nucleotides with only 10 pmoles of nucleotides required. The amounts of 8-OH-dG in DNA detected by the postlabeling method correlated well with the electrochemical detection method but were consistently lower.

Original languageEnglish (US)
Pages (from-to)1880-1882
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume39
Issue number7
StatePublished - 1991
Externally publishedYes

Fingerprint

DNA
Nucleotides
8-oxo-7-hydrodeoxyguanosine
hydroxide ion
2'-deoxyguanosine 3'-phosphate

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Detection of 8-hydroxy-2'-deoxyguanosine in deoxyribonucleic acid by the 32P-postlabeling method. / Lu, Leejane; Tasaka, F.; Hokanson, J. A.; Kohda, K.

In: Chemical and Pharmaceutical Bulletin, Vol. 39, No. 7, 1991, p. 1880-1882.

Research output: Contribution to journalArticle

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