Detection of a novel rickettsia from leptotrombidium scutellare mites (Acari: Trombiculidae) From Shandong of China

Yuting Huang, Li Zhao, Zhentang Zhang, Miaomiao Liu, Zaifeng Xue, Dongqiang Ma, Xifeng Sun, Yue Sun, Chuanmin Zhou, Xiangrong Qin, Yelei Zhu, Wenqian Li, Hao Yu, Xue Jie Yu, Kevin Macaluso

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Leptotrombidium scutellare mites, the vector of Orientia tsutsugamushi, have rarely been reported to associate with Rickettsia species. Three hundred nineteen chiggers were collected from the ears of 32 rodents captured in Huangdao District of Qingdao City, China, in October 2015. The chigger samples were tested for Rickettsia, severe fever with thrombocytopenia syndrome virus, and hantavirus by PCR or RT-PCR amplification. All mites were classified morphologically and molecularly as L. scutellare chiggers. Rickettsial DNA sequences were amplified for four genes including 16S rRNA, ompB, gltA, and 17 kD protein genes. The minimum infection rate (MIR; number of positive pools/total specimens tested) of the Rickettsia species in the chiggers were 2.8% (9/319). Phylogenetic analysis indicated that individual genes were closely related to different Rickettsia species including R. felis (with 16S rRNA gene), R. australis (with gltA gene), an unnamed Rickettsia sp. TwKM02 (with ompB gene), and Rickettsia endosymbiont of soft tick Ornithodoros erraticus (with 17 kD protein gene). Phylogenic analysis of the concatenated sequence of 16S rRNA, gltA, ompB, and 17 kD protein genes indicated that the Rickettsia species from L. scutellare chigger was most closely related to R. australis and R. akari. These results indicated that the Rickettsia species in chiggers was unique; it was named Candidatus Rickettsia leptotrombidium. Severe fever with thrombocytopenia syndrome virus and hantavirus were not amplified from the chiggers, suggesting lack of infection of these pathogens in the chiggers. A unique Rickettsia species was detected in L. scutellare, which expanded the knowledge on the vector distribution of Rickettsia.

Original languageEnglish (US)
Pages (from-to)544-549
Number of pages6
JournalJournal of Medical Entomology
Volume54
Issue number3
DOIs
StatePublished - May 1 2017

Fingerprint

Leptotrombidium
Trombiculidae
Rickettsia
Mites
chiggers
mites
Acari
China
genes
Hantavirus
thrombocytopenia
ribosomal RNA
Thrombocytopenia
Genes
fever
Ornithodoros erraticus
Fever
Orientia tsutsugamushi
Argasidae
Ornithodoros

Keywords

  • Candidatus Rickettsia leptotrombidium
  • Chigger
  • Leptotrombidium scutellare
  • Rickettsia
  • SFTSV

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Detection of a novel rickettsia from leptotrombidium scutellare mites (Acari : Trombiculidae) From Shandong of China. / Huang, Yuting; Zhao, Li; Zhang, Zhentang; Liu, Miaomiao; Xue, Zaifeng; Ma, Dongqiang; Sun, Xifeng; Sun, Yue; Zhou, Chuanmin; Qin, Xiangrong; Zhu, Yelei; Li, Wenqian; Yu, Hao; Yu, Xue Jie; Macaluso, Kevin.

In: Journal of Medical Entomology, Vol. 54, No. 3, 01.05.2017, p. 544-549.

Research output: Contribution to journalArticle

Huang, Y, Zhao, L, Zhang, Z, Liu, M, Xue, Z, Ma, D, Sun, X, Sun, Y, Zhou, C, Qin, X, Zhu, Y, Li, W, Yu, H, Yu, XJ & Macaluso, K 2017, 'Detection of a novel rickettsia from leptotrombidium scutellare mites (Acari: Trombiculidae) From Shandong of China', Journal of Medical Entomology, vol. 54, no. 3, pp. 544-549. https://doi.org/10.1093/jme/tjw234
Huang, Yuting ; Zhao, Li ; Zhang, Zhentang ; Liu, Miaomiao ; Xue, Zaifeng ; Ma, Dongqiang ; Sun, Xifeng ; Sun, Yue ; Zhou, Chuanmin ; Qin, Xiangrong ; Zhu, Yelei ; Li, Wenqian ; Yu, Hao ; Yu, Xue Jie ; Macaluso, Kevin. / Detection of a novel rickettsia from leptotrombidium scutellare mites (Acari : Trombiculidae) From Shandong of China. In: Journal of Medical Entomology. 2017 ; Vol. 54, No. 3. pp. 544-549.
@article{43b70a8cc94a4932ad6691eff08b7d87,
title = "Detection of a novel rickettsia from leptotrombidium scutellare mites (Acari: Trombiculidae) From Shandong of China",
abstract = "Leptotrombidium scutellare mites, the vector of Orientia tsutsugamushi, have rarely been reported to associate with Rickettsia species. Three hundred nineteen chiggers were collected from the ears of 32 rodents captured in Huangdao District of Qingdao City, China, in October 2015. The chigger samples were tested for Rickettsia, severe fever with thrombocytopenia syndrome virus, and hantavirus by PCR or RT-PCR amplification. All mites were classified morphologically and molecularly as L. scutellare chiggers. Rickettsial DNA sequences were amplified for four genes including 16S rRNA, ompB, gltA, and 17 kD protein genes. The minimum infection rate (MIR; number of positive pools/total specimens tested) of the Rickettsia species in the chiggers were 2.8{\%} (9/319). Phylogenetic analysis indicated that individual genes were closely related to different Rickettsia species including R. felis (with 16S rRNA gene), R. australis (with gltA gene), an unnamed Rickettsia sp. TwKM02 (with ompB gene), and Rickettsia endosymbiont of soft tick Ornithodoros erraticus (with 17 kD protein gene). Phylogenic analysis of the concatenated sequence of 16S rRNA, gltA, ompB, and 17 kD protein genes indicated that the Rickettsia species from L. scutellare chigger was most closely related to R. australis and R. akari. These results indicated that the Rickettsia species in chiggers was unique; it was named Candidatus Rickettsia leptotrombidium. Severe fever with thrombocytopenia syndrome virus and hantavirus were not amplified from the chiggers, suggesting lack of infection of these pathogens in the chiggers. A unique Rickettsia species was detected in L. scutellare, which expanded the knowledge on the vector distribution of Rickettsia.",
keywords = "Candidatus Rickettsia leptotrombidium, Chigger, Leptotrombidium scutellare, Rickettsia, SFTSV",
author = "Yuting Huang and Li Zhao and Zhentang Zhang and Miaomiao Liu and Zaifeng Xue and Dongqiang Ma and Xifeng Sun and Yue Sun and Chuanmin Zhou and Xiangrong Qin and Yelei Zhu and Wenqian Li and Hao Yu and Yu, {Xue Jie} and Kevin Macaluso",
year = "2017",
month = "5",
day = "1",
doi = "10.1093/jme/tjw234",
language = "English (US)",
volume = "54",
pages = "544--549",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "3",

}

TY - JOUR

T1 - Detection of a novel rickettsia from leptotrombidium scutellare mites (Acari

T2 - Trombiculidae) From Shandong of China

AU - Huang, Yuting

AU - Zhao, Li

AU - Zhang, Zhentang

AU - Liu, Miaomiao

AU - Xue, Zaifeng

AU - Ma, Dongqiang

AU - Sun, Xifeng

AU - Sun, Yue

AU - Zhou, Chuanmin

AU - Qin, Xiangrong

AU - Zhu, Yelei

AU - Li, Wenqian

AU - Yu, Hao

AU - Yu, Xue Jie

AU - Macaluso, Kevin

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Leptotrombidium scutellare mites, the vector of Orientia tsutsugamushi, have rarely been reported to associate with Rickettsia species. Three hundred nineteen chiggers were collected from the ears of 32 rodents captured in Huangdao District of Qingdao City, China, in October 2015. The chigger samples were tested for Rickettsia, severe fever with thrombocytopenia syndrome virus, and hantavirus by PCR or RT-PCR amplification. All mites were classified morphologically and molecularly as L. scutellare chiggers. Rickettsial DNA sequences were amplified for four genes including 16S rRNA, ompB, gltA, and 17 kD protein genes. The minimum infection rate (MIR; number of positive pools/total specimens tested) of the Rickettsia species in the chiggers were 2.8% (9/319). Phylogenetic analysis indicated that individual genes were closely related to different Rickettsia species including R. felis (with 16S rRNA gene), R. australis (with gltA gene), an unnamed Rickettsia sp. TwKM02 (with ompB gene), and Rickettsia endosymbiont of soft tick Ornithodoros erraticus (with 17 kD protein gene). Phylogenic analysis of the concatenated sequence of 16S rRNA, gltA, ompB, and 17 kD protein genes indicated that the Rickettsia species from L. scutellare chigger was most closely related to R. australis and R. akari. These results indicated that the Rickettsia species in chiggers was unique; it was named Candidatus Rickettsia leptotrombidium. Severe fever with thrombocytopenia syndrome virus and hantavirus were not amplified from the chiggers, suggesting lack of infection of these pathogens in the chiggers. A unique Rickettsia species was detected in L. scutellare, which expanded the knowledge on the vector distribution of Rickettsia.

AB - Leptotrombidium scutellare mites, the vector of Orientia tsutsugamushi, have rarely been reported to associate with Rickettsia species. Three hundred nineteen chiggers were collected from the ears of 32 rodents captured in Huangdao District of Qingdao City, China, in October 2015. The chigger samples were tested for Rickettsia, severe fever with thrombocytopenia syndrome virus, and hantavirus by PCR or RT-PCR amplification. All mites were classified morphologically and molecularly as L. scutellare chiggers. Rickettsial DNA sequences were amplified for four genes including 16S rRNA, ompB, gltA, and 17 kD protein genes. The minimum infection rate (MIR; number of positive pools/total specimens tested) of the Rickettsia species in the chiggers were 2.8% (9/319). Phylogenetic analysis indicated that individual genes were closely related to different Rickettsia species including R. felis (with 16S rRNA gene), R. australis (with gltA gene), an unnamed Rickettsia sp. TwKM02 (with ompB gene), and Rickettsia endosymbiont of soft tick Ornithodoros erraticus (with 17 kD protein gene). Phylogenic analysis of the concatenated sequence of 16S rRNA, gltA, ompB, and 17 kD protein genes indicated that the Rickettsia species from L. scutellare chigger was most closely related to R. australis and R. akari. These results indicated that the Rickettsia species in chiggers was unique; it was named Candidatus Rickettsia leptotrombidium. Severe fever with thrombocytopenia syndrome virus and hantavirus were not amplified from the chiggers, suggesting lack of infection of these pathogens in the chiggers. A unique Rickettsia species was detected in L. scutellare, which expanded the knowledge on the vector distribution of Rickettsia.

KW - Candidatus Rickettsia leptotrombidium

KW - Chigger

KW - Leptotrombidium scutellare

KW - Rickettsia

KW - SFTSV

UR - http://www.scopus.com/inward/record.url?scp=85026353052&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026353052&partnerID=8YFLogxK

U2 - 10.1093/jme/tjw234

DO - 10.1093/jme/tjw234

M3 - Article

C2 - 28399204

AN - SCOPUS:85026353052

VL - 54

SP - 544

EP - 549

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 3

ER -