Detection of a spotted fever group Rickettsia in the tick Haemaphysalis juxtakochi in Rondonia, Brazil

Marcelo B. Labruna, Luis Marcelo A Camargo, Erney P. Camargo, David Walker

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The tick genus Haemaphysalis is represented by four species in the New World, of which only the species Haemaphysalis leporispalustris has been associated with Rickettsiae. The present study reports for the first time the presence of a Rickettsia strain in the tick Haemaphysalis juxtakochi. A free-living male of H. juxtakochi, collected in the state of Rondonia, Western Amazon, Brazil, was subjected to DNA extraction and tested by PCR targeting the four rickettsial genes: gltA, 17-kDa, ompA and ompB. The nucleotide sequences obtained from the PCR products were, by BLAST analyses, closest to Rickettsia rhipicephali sharing 99.7% (1147/1150), 98.8% (429/434), 99.0% (486/491) and 99.0% (809/817) identities with the partial sequences of the gltA, 17-kDa, ompA and ompB genes, respectively. Phylogenetic analyses inferred from the four rickettsial genes showed a high-degree of similarity of this H. juxtakochi-Rickettsia with R. rhipicephali. These two agents grouped together in all trees, always with high bootstrap support (75-96%). This study gives molecular evidence for the presence of a Rickettsia species, designated as strain R300, in the tick H. juxtakochi from the Western Amazon area of Brazil. Genetic analyses showed R300 to be closely related to R. rhipicephali.

Original languageEnglish (US)
Pages (from-to)169-174
Number of pages6
JournalVeterinary Parasitology
Volume127
Issue number2
DOIs
StatePublished - Jan 20 2005
Externally publishedYes

Fingerprint

Rickettsia rhipicephali
Haemaphysalis
Rickettsia
Ticks
fever
Brazil
ticks
Fever
Haemaphysalis leporispalustris
genes
Genes
Polymerase Chain Reaction
nucleotide sequences
phylogeny
DNA

Keywords

  • Amazon
  • Brazil
  • Haemaphysalis juxtakochi
  • Rickettsia
  • Ticks

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

Cite this

Detection of a spotted fever group Rickettsia in the tick Haemaphysalis juxtakochi in Rondonia, Brazil. / Labruna, Marcelo B.; Camargo, Luis Marcelo A; Camargo, Erney P.; Walker, David.

In: Veterinary Parasitology, Vol. 127, No. 2, 20.01.2005, p. 169-174.

Research output: Contribution to journalArticle

Labruna, Marcelo B. ; Camargo, Luis Marcelo A ; Camargo, Erney P. ; Walker, David. / Detection of a spotted fever group Rickettsia in the tick Haemaphysalis juxtakochi in Rondonia, Brazil. In: Veterinary Parasitology. 2005 ; Vol. 127, No. 2. pp. 169-174.
@article{c9bfe9dd1fda46e08392157549eb37eb,
title = "Detection of a spotted fever group Rickettsia in the tick Haemaphysalis juxtakochi in Rondonia, Brazil",
abstract = "The tick genus Haemaphysalis is represented by four species in the New World, of which only the species Haemaphysalis leporispalustris has been associated with Rickettsiae. The present study reports for the first time the presence of a Rickettsia strain in the tick Haemaphysalis juxtakochi. A free-living male of H. juxtakochi, collected in the state of Rondonia, Western Amazon, Brazil, was subjected to DNA extraction and tested by PCR targeting the four rickettsial genes: gltA, 17-kDa, ompA and ompB. The nucleotide sequences obtained from the PCR products were, by BLAST analyses, closest to Rickettsia rhipicephali sharing 99.7{\%} (1147/1150), 98.8{\%} (429/434), 99.0{\%} (486/491) and 99.0{\%} (809/817) identities with the partial sequences of the gltA, 17-kDa, ompA and ompB genes, respectively. Phylogenetic analyses inferred from the four rickettsial genes showed a high-degree of similarity of this H. juxtakochi-Rickettsia with R. rhipicephali. These two agents grouped together in all trees, always with high bootstrap support (75-96{\%}). This study gives molecular evidence for the presence of a Rickettsia species, designated as strain R300, in the tick H. juxtakochi from the Western Amazon area of Brazil. Genetic analyses showed R300 to be closely related to R. rhipicephali.",
keywords = "Amazon, Brazil, Haemaphysalis juxtakochi, Rickettsia, Ticks",
author = "Labruna, {Marcelo B.} and Camargo, {Luis Marcelo A} and Camargo, {Erney P.} and David Walker",
year = "2005",
month = "1",
day = "20",
doi = "10.1016/j.vetpar.2004.09.024",
language = "English (US)",
volume = "127",
pages = "169--174",
journal = "Veterinary Parasitology: Regional Studies and Reports",
issn = "0304-4017",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Detection of a spotted fever group Rickettsia in the tick Haemaphysalis juxtakochi in Rondonia, Brazil

AU - Labruna, Marcelo B.

AU - Camargo, Luis Marcelo A

AU - Camargo, Erney P.

AU - Walker, David

PY - 2005/1/20

Y1 - 2005/1/20

N2 - The tick genus Haemaphysalis is represented by four species in the New World, of which only the species Haemaphysalis leporispalustris has been associated with Rickettsiae. The present study reports for the first time the presence of a Rickettsia strain in the tick Haemaphysalis juxtakochi. A free-living male of H. juxtakochi, collected in the state of Rondonia, Western Amazon, Brazil, was subjected to DNA extraction and tested by PCR targeting the four rickettsial genes: gltA, 17-kDa, ompA and ompB. The nucleotide sequences obtained from the PCR products were, by BLAST analyses, closest to Rickettsia rhipicephali sharing 99.7% (1147/1150), 98.8% (429/434), 99.0% (486/491) and 99.0% (809/817) identities with the partial sequences of the gltA, 17-kDa, ompA and ompB genes, respectively. Phylogenetic analyses inferred from the four rickettsial genes showed a high-degree of similarity of this H. juxtakochi-Rickettsia with R. rhipicephali. These two agents grouped together in all trees, always with high bootstrap support (75-96%). This study gives molecular evidence for the presence of a Rickettsia species, designated as strain R300, in the tick H. juxtakochi from the Western Amazon area of Brazil. Genetic analyses showed R300 to be closely related to R. rhipicephali.

AB - The tick genus Haemaphysalis is represented by four species in the New World, of which only the species Haemaphysalis leporispalustris has been associated with Rickettsiae. The present study reports for the first time the presence of a Rickettsia strain in the tick Haemaphysalis juxtakochi. A free-living male of H. juxtakochi, collected in the state of Rondonia, Western Amazon, Brazil, was subjected to DNA extraction and tested by PCR targeting the four rickettsial genes: gltA, 17-kDa, ompA and ompB. The nucleotide sequences obtained from the PCR products were, by BLAST analyses, closest to Rickettsia rhipicephali sharing 99.7% (1147/1150), 98.8% (429/434), 99.0% (486/491) and 99.0% (809/817) identities with the partial sequences of the gltA, 17-kDa, ompA and ompB genes, respectively. Phylogenetic analyses inferred from the four rickettsial genes showed a high-degree of similarity of this H. juxtakochi-Rickettsia with R. rhipicephali. These two agents grouped together in all trees, always with high bootstrap support (75-96%). This study gives molecular evidence for the presence of a Rickettsia species, designated as strain R300, in the tick H. juxtakochi from the Western Amazon area of Brazil. Genetic analyses showed R300 to be closely related to R. rhipicephali.

KW - Amazon

KW - Brazil

KW - Haemaphysalis juxtakochi

KW - Rickettsia

KW - Ticks

UR - http://www.scopus.com/inward/record.url?scp=20344388543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20344388543&partnerID=8YFLogxK

U2 - 10.1016/j.vetpar.2004.09.024

DO - 10.1016/j.vetpar.2004.09.024

M3 - Article

VL - 127

SP - 169

EP - 174

JO - Veterinary Parasitology: Regional Studies and Reports

JF - Veterinary Parasitology: Regional Studies and Reports

SN - 0304-4017

IS - 2

ER -