Detection of airborne Stachybotrys chartarum macrocyclic trichothecene mycotoxins on particulates smaller than conidia

T. L. Brasel, D. R. Douglas, S. C. Wilson, D. C. Straus

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Highly respirable particles (diameter, < 1 μm) constitute the majority of particulate matter found in indoor air. It is hypothesized that these particles serve as carriers for toxic compounds, specifically the compounds produced by molds in water-damaged buildings. The presence off airborne Stachybotrys chartarum trichothecene mycotoxins on particles smaller than conidia (e.g., fungal fragments) was therefore investigated. Cellulose ceiling tiles with confluent Stachybotrys growth were placed in gas-drying containers through which filtered air was passed. Exiting particulates were collected by using a series of polycarbonate membrane filters with decreasing pore sizes. Scanning electron microscopy was employed to determine the presence of conidia on the filters. A competitive enzyme-linked immunosorbent assay (ELISA) specific for macrocyclic trichothecenes was used to analyze filter extracts. Cross-reactivity to various mycotoxins was examined to confirm the specificity. Statistically significant (P < 0.05) ELISA binding was observed primarily for macrocyclic trichothecenes at concentrations of 50 and 5 ng/ml and 500 pg/ml (58.4 to 83.5% inhibition). Of the remaining toxins tested, only verrucarol and diacetylverrucarol (nonmacrocyclic trichothecenes) demonstrated significant binding (18.2 and 51.7% inhibition, respectively) and then only at high concentrations. The results showed that extracts from conidium-free filters demonstrated statistically significant (P < 0.05) antibody binding that increased with sampling time (38.4 to 71.9% inhibition, representing a range of 0.5 to 4.0 ng/ml). High-performance liquid chromatography analysis suggested the presence of satratoxin H in conidium-free filter extracts. These data show that S. chartarum trichothecene mycotoxins can become airborne in association with intact conidia or smaller particles. These findings may have important implications for indoor air quality assessment.

Original languageEnglish (US)
Pages (from-to)114-122
Number of pages9
JournalApplied and Environmental Microbiology
Volume71
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

Stachybotrys chartarum
Stachybotrys
Trichothecenes
Fungal Spores
trichothecenes
Mycotoxins
mycotoxins
conidia
particulates
filter
polycarbonate
indoor air
extracts
Enzyme-Linked Immunosorbent Assay
Air
enzyme-linked immunosorbent assay
assay
enzyme
Indoor Air Pollution
air

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Detection of airborne Stachybotrys chartarum macrocyclic trichothecene mycotoxins on particulates smaller than conidia. / Brasel, T. L.; Douglas, D. R.; Wilson, S. C.; Straus, D. C.

In: Applied and Environmental Microbiology, Vol. 71, No. 1, 01.2005, p. 114-122.

Research output: Contribution to journalArticle

Brasel, T. L. ; Douglas, D. R. ; Wilson, S. C. ; Straus, D. C. / Detection of airborne Stachybotrys chartarum macrocyclic trichothecene mycotoxins on particulates smaller than conidia. In: Applied and Environmental Microbiology. 2005 ; Vol. 71, No. 1. pp. 114-122.
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