Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay

Udeni B R Balasuriya, Pei-Yong Shi, Susan J. Wong, Valerie L. Demarest, Ian A. Gardner, Pamela J. Hullinger, Gregory L. Ferraro, Joshua D. Boone, Casey L. De Cino, Amy L. Glaser, Randall W. Renshaw, Michel Ledizet, Raymond A. Koski, N. James MacLachlan

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

One hundred and ninety-one sera from horses that recently were exposed to West Nile virus (WNV) by either vaccination or natural infection or that were not vaccinated and remained free of infection were used to evaluate fluorescent microsphere immunoassays (MIAs) incorporating recombinant WNV envelope protein (rE) and recombinant nonstructural proteins (rNS1, rNS3, and rNS5) for detection of equine antibodies to WNV. The rE MIA had a diagnostic sensitivity and specificity, respectively, of 99.3% and 97.4% for detection of WNV antibodies in the serum of horses that were recently vaccinated or naturally infected with WNV, as compared to the plaque reduction neutralization test (PRNT). The positive rE MIA results were assumed to be WNV-specific because of the close agreement between this assay and the PRNT and the fact that unvaccinated control horses included in this study were confirmed to be free of exposure to the related St Louis encephalitis virus. The NS protein-based MIA were all less sensitive than either the rE MIA or PRNT (sensitivity 0-48.0), although the rNS1 MIA distinguished horses vaccinated with the recombinant WNV vaccine from those that were immunized with the inactivated WNV vaccine (P <0.0001) or naturally infected with WNV (P <0.0001). The rE MIA would appear to provide a rapid, convenient, inexpensive, and accurate test for the screening of equine sera for the presence of antibodies to WNV.

Original languageEnglish (US)
Pages (from-to)392-395
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume18
Issue number4
StatePublished - Jul 2006
Externally publishedYes

Fingerprint

West Nile virus
antibody detection
immunoassays
Microspheres
Immunoassay
Horses
horses
Antibodies
Serum
Neutralization Tests
West Nile Virus Vaccines
neutralization tests
Encephalitis Viruses
antibodies
Viral Envelope Proteins
Inactivated Vaccines
Synthetic Vaccines
Saint Louis encephalitis virus
vaccines
diagnostic specificity

Keywords

  • Horse
  • Microsphere immunoassay
  • Serology
  • West Nile virus

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Balasuriya, U. B. R., Shi, P-Y., Wong, S. J., Demarest, V. L., Gardner, I. A., Hullinger, P. J., ... MacLachlan, N. J. (2006). Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay. Journal of Veterinary Diagnostic Investigation, 18(4), 392-395.

Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay. / Balasuriya, Udeni B R; Shi, Pei-Yong; Wong, Susan J.; Demarest, Valerie L.; Gardner, Ian A.; Hullinger, Pamela J.; Ferraro, Gregory L.; Boone, Joshua D.; De Cino, Casey L.; Glaser, Amy L.; Renshaw, Randall W.; Ledizet, Michel; Koski, Raymond A.; MacLachlan, N. James.

In: Journal of Veterinary Diagnostic Investigation, Vol. 18, No. 4, 07.2006, p. 392-395.

Research output: Contribution to journalArticle

Balasuriya, UBR, Shi, P-Y, Wong, SJ, Demarest, VL, Gardner, IA, Hullinger, PJ, Ferraro, GL, Boone, JD, De Cino, CL, Glaser, AL, Renshaw, RW, Ledizet, M, Koski, RA & MacLachlan, NJ 2006, 'Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay', Journal of Veterinary Diagnostic Investigation, vol. 18, no. 4, pp. 392-395.
Balasuriya UBR, Shi P-Y, Wong SJ, Demarest VL, Gardner IA, Hullinger PJ et al. Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay. Journal of Veterinary Diagnostic Investigation. 2006 Jul;18(4):392-395.
Balasuriya, Udeni B R ; Shi, Pei-Yong ; Wong, Susan J. ; Demarest, Valerie L. ; Gardner, Ian A. ; Hullinger, Pamela J. ; Ferraro, Gregory L. ; Boone, Joshua D. ; De Cino, Casey L. ; Glaser, Amy L. ; Renshaw, Randall W. ; Ledizet, Michel ; Koski, Raymond A. ; MacLachlan, N. James. / Detection of antibodies to West Nile virus in equine sera using microsphere immunoassay. In: Journal of Veterinary Diagnostic Investigation. 2006 ; Vol. 18, No. 4. pp. 392-395.
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