Detection of antigenemia by enzyme-linked immunosorbent assay in horses with experimental Ehrlichia risticii infection.

R. E. Corstvet, S. D. Gaunt, P. A. Karns, D. Burgermeister, Jere McBride, S. M. Nicholson, R. A. Battistini

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Four horses were inoculated with Ehrlichia risticii contained in either infected murine P388 D1 cells or heparinized blood from an infected horse. All 4 horses produced serum antibody, plasma antigen, and clinical signs of the disease. An enzyme-linked immunosorbent assay was used to detect antibody in the serum and was also used in conjunction with an anti-E. risticii monoclonal antibody to detect antigenemia. These laboratory and clinical findings were correlated to determine the efficiency of the antigen detection method for discerning E. risticii infection.

Original languageEnglish (US)
Pages (from-to)33-36
Number of pages4
JournalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Volume5
Issue number1
StatePublished - Jan 1993
Externally publishedYes

Fingerprint

Neorickettsia risticii
Horses
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
blood serum
horses
Infection
infection
Antigens
antigen detection
antibodies
Antibodies
Serum
monoclonal antibodies
Monoclonal Antibodies
antigens
blood
mice
cells

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Detection of antigenemia by enzyme-linked immunosorbent assay in horses with experimental Ehrlichia risticii infection. / Corstvet, R. E.; Gaunt, S. D.; Karns, P. A.; Burgermeister, D.; McBride, Jere; Nicholson, S. M.; Battistini, R. A.

In: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Vol. 5, No. 1, 01.1993, p. 33-36.

Research output: Contribution to journalArticle

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