Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis

Tais Saito, A. A. Alfieri, S. R. Wosiacki, F. J. Negrão, H. S A Morais, A. F. Alfieri

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RT-PCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs.

Original languageEnglish (US)
Pages (from-to)116-119
Number of pages4
JournalResearch in Veterinary Science
Volume80
Issue number1
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Distemper
Canine Distemper Virus
distemper
Canine distemper virus
Encephalitis
encephalitis
Reverse Transcriptase Polymerase Chain Reaction
urine
reverse transcriptase polymerase chain reaction
Urine
Dogs
dogs
leukocytes
Leukocytes
nucleoproteins
Nucleoproteins
body fluids
Body Fluids
cerebrospinal fluid
blood serum

Keywords

  • Canine distemper
  • Canine distemper virus
  • Dogs
  • RT-PCR
  • Urine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis. / Saito, Tais; Alfieri, A. A.; Wosiacki, S. R.; Negrão, F. J.; Morais, H. S A; Alfieri, A. F.

In: Research in Veterinary Science, Vol. 80, No. 1, 02.2006, p. 116-119.

Research output: Contribution to journalArticle

Saito, Tais ; Alfieri, A. A. ; Wosiacki, S. R. ; Negrão, F. J. ; Morais, H. S A ; Alfieri, A. F. / Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis. In: Research in Veterinary Science. 2006 ; Vol. 80, No. 1. pp. 116-119.
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