Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction

M. J. Loeffelholz, C. A. Lewinski, S. R. Silver, A. P. Purohit, S. A. Herman, D. A. Buonagurio, E. A. Dragon

Research output: Contribution to journalArticle

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Abstract

A rapid and sensitive polymerase chain reaction (PCR)-based assay for detection of Chlamydia trachomatis in cervical specimens is described. This assay consists of (i) sample preparation which avoids the use of heat, centrifugation, or organic extractions; (ii) rapid, two-temperature PCR amplification of C. trachomatis cryptic plasmid sequences; and (iii) capture and colorimetric detection of amplified DNA in microwell plates. PCR was compared with culture by using 503 cervical specimens. After resolution of discrepant specimens with a confirmatory PCR assay directed against the chlamydial major outer membrane protein gene, PCR had a sensitivity of 97% and a specificity of 99.7% while culture had a sensitivity of 85.7% and a specificity of 100%. In a separate study, PCR was compared with a direct specimen enzyme immunoassay (Chlamydiazyme; Abbott Diagnostics) by using 375 cervical specimens. After resolution of discrepant specimens, PCR had a sensitivity and specificity of 100%, while the enzyme immunoassay had a sensitivity of 58.8% and a specificity of 100%.

Original languageEnglish (US)
Pages (from-to)2847-2851
Number of pages5
JournalJournal of Clinical Microbiology
Volume30
Issue number11
StatePublished - 1992
Externally publishedYes

Fingerprint

Chlamydia trachomatis
Polymerase Chain Reaction
Immunoenzyme Techniques
Centrifugation
Membrane Proteins
Plasmids
Hot Temperature
Sensitivity and Specificity
Temperature
DNA
Genes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Loeffelholz, M. J., Lewinski, C. A., Silver, S. R., Purohit, A. P., Herman, S. A., Buonagurio, D. A., & Dragon, E. A. (1992). Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. Journal of Clinical Microbiology, 30(11), 2847-2851.

Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. / Loeffelholz, M. J.; Lewinski, C. A.; Silver, S. R.; Purohit, A. P.; Herman, S. A.; Buonagurio, D. A.; Dragon, E. A.

In: Journal of Clinical Microbiology, Vol. 30, No. 11, 1992, p. 2847-2851.

Research output: Contribution to journalArticle

Loeffelholz, MJ, Lewinski, CA, Silver, SR, Purohit, AP, Herman, SA, Buonagurio, DA & Dragon, EA 1992, 'Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction', Journal of Clinical Microbiology, vol. 30, no. 11, pp. 2847-2851.
Loeffelholz MJ, Lewinski CA, Silver SR, Purohit AP, Herman SA, Buonagurio DA et al. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. Journal of Clinical Microbiology. 1992;30(11):2847-2851.
Loeffelholz, M. J. ; Lewinski, C. A. ; Silver, S. R. ; Purohit, A. P. ; Herman, S. A. ; Buonagurio, D. A. ; Dragon, E. A. / Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. In: Journal of Clinical Microbiology. 1992 ; Vol. 30, No. 11. pp. 2847-2851.
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