Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein

M. Niikura, Tetsuro Ikegami, M. Saijo, I. Kurane, M. E. Miranda, S. Morikawa

Research output: Contribution to journalArticle

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Abstract

With the increase in international traffic, the risk of introducing rare but severe infectious diseases like Ebola hemorrhagic fever is increasing all over the world. However, the system for the diagnosis of Ebola virus infection is available in a limited number of countries. In the present study, we developed an Ebola virus antigen-detection enzyme-linked immunosorbent assay (ELISA) system using a novel monoclonal antibody (MAb) to the nucleoprotein (NP). This antibody recognized an epitope defined by a 26-amino-acid stretch near the C terminus of NP. In a sandwich ELISA system with the MAb, as little as 30 ng of purified recombinant NP (rNP) was detected. Although this MAb was prepared by immunization with rNP of subtype Zaire, it also reacted to the corresponding region of NP derived from the Reston and Sudan subtypes. These results suggest that our ELISA system should work with three of four Ebola subtypes. Furthermore, our ELISA system detected the NP in subtype Reston-infected monkey specimens, while the background level in noninfected specimens was very low, suggesting the usefulness of the ELISA for laboratory diagnosis with clinical specimens.

Original languageEnglish (US)
Pages (from-to)3267-3271
Number of pages5
JournalJournal of Clinical Microbiology
Volume39
Issue number9
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Nucleoproteins
Viral Antigens
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Ebola Hemorrhagic Fever
Ebolavirus
Democratic Republic of the Congo
Sudan
Clinical Laboratory Techniques
Haplorhini
Communicable Diseases
Epitopes
Immunization
Antigens
Amino Acids
Antibodies

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein. / Niikura, M.; Ikegami, Tetsuro; Saijo, M.; Kurane, I.; Miranda, M. E.; Morikawa, S.

In: Journal of Clinical Microbiology, Vol. 39, No. 9, 2001, p. 3267-3271.

Research output: Contribution to journalArticle

Niikura, M. ; Ikegami, Tetsuro ; Saijo, M. ; Kurane, I. ; Miranda, M. E. ; Morikawa, S. / Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein. In: Journal of Clinical Microbiology. 2001 ; Vol. 39, No. 9. pp. 3267-3271.
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