Detection of Ehrlichia chaffeensis in human tissue by using a species- specific monoclonal antibody

X. Yu, P. Brouqui, J. S. Dumler, D. Raoult

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A mouse monoclonal antibody (MAb 1A9) was produced and used in detection of Ehrlichia chaffeensis in human tissues including kidney, liver, and lung by using an indirect immunohistologic stain. MAb 1A9 was specific to E. chaffeensis and did not react with other bacteria, including Ehrlichia canis, which is the organism most closely related to E. chaffeensis. It reacted with an epitope present in two surface proteins of E. chaffeensis with molecular masses of 29 and 27 kDa. E. chaffeensis was easily detected in human tissue by immunohistology with MAb 1A9. This study demonstrates that our MAb can provide a specific and simple method for detection of E. chaffeensis in clinical specimens for establishing an etiologic diagnosis of human ehrlichiosis; it may also provide a tool for the investigation of immunopathologic characteristics in infected patients.

Original languageEnglish (US)
Pages (from-to)3284-3288
Number of pages5
JournalJournal of Clinical Microbiology
Volume31
Issue number12
StatePublished - 1993
Externally publishedYes

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Ehrlichia chaffeensis
Monoclonal Antibodies
Ehrlichia canis
Ehrlichiosis
Epitopes
Membrane Proteins
Coloring Agents
Bacteria
Kidney
Lung
Liver

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Detection of Ehrlichia chaffeensis in human tissue by using a species- specific monoclonal antibody. / Yu, X.; Brouqui, P.; Dumler, J. S.; Raoult, D.

In: Journal of Clinical Microbiology, Vol. 31, No. 12, 1993, p. 3284-3288.

Research output: Contribution to journalArticle

Yu, X. ; Brouqui, P. ; Dumler, J. S. ; Raoult, D. / Detection of Ehrlichia chaffeensis in human tissue by using a species- specific monoclonal antibody. In: Journal of Clinical Microbiology. 1993 ; Vol. 31, No. 12. pp. 3284-3288.
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