Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children.

Y. Okamoto, K. Kudo, K. Shirotori, M. Nakazawa, E. Ito, K. Togawa, Janak Patel, P. L. Ogra

Research output: Contribution to journalArticle

Abstract

The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

Original languageEnglish (US)
Pages (from-to)7-10
Number of pages4
JournalThe Annals of otology, rhinology & laryngology. Supplement
Volume157
StatePublished - Oct 1992
Externally publishedYes

Fingerprint

Otitis Media with Effusion
Respiratory Syncytial Viruses
Respiratory Syncytial Virus Infections
Nasopharynx
Otitis Media
Reverse Transcriptase Polymerase Chain Reaction
Disease Outbreaks
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Okamoto, Y., Kudo, K., Shirotori, K., Nakazawa, M., Ito, E., Togawa, K., ... Ogra, P. L. (1992). Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children. The Annals of otology, rhinology & laryngology. Supplement, 157, 7-10.

Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children. / Okamoto, Y.; Kudo, K.; Shirotori, K.; Nakazawa, M.; Ito, E.; Togawa, K.; Patel, Janak; Ogra, P. L.

In: The Annals of otology, rhinology & laryngology. Supplement, Vol. 157, 10.1992, p. 7-10.

Research output: Contribution to journalArticle

Okamoto, Y, Kudo, K, Shirotori, K, Nakazawa, M, Ito, E, Togawa, K, Patel, J & Ogra, PL 1992, 'Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children.', The Annals of otology, rhinology & laryngology. Supplement, vol. 157, pp. 7-10.
Okamoto, Y. ; Kudo, K. ; Shirotori, K. ; Nakazawa, M. ; Ito, E. ; Togawa, K. ; Patel, Janak ; Ogra, P. L. / Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children. In: The Annals of otology, rhinology & laryngology. Supplement. 1992 ; Vol. 157. pp. 7-10.
@article{8cd960e055984d44a8359ab7395f7338,
title = "Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children.",
abstract = "The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75{\%}) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.",
author = "Y. Okamoto and K. Kudo and K. Shirotori and M. Nakazawa and E. Ito and K. Togawa and Janak Patel and Ogra, {P. L.}",
year = "1992",
month = "10",
language = "English (US)",
volume = "157",
pages = "7--10",
journal = "The Annals of otology, rhinology & laryngology. Supplement",
issn = "0096-8056",
publisher = "Annals Publishing Company",

}

TY - JOUR

T1 - Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children.

AU - Okamoto, Y.

AU - Kudo, K.

AU - Shirotori, K.

AU - Nakazawa, M.

AU - Ito, E.

AU - Togawa, K.

AU - Patel, Janak

AU - Ogra, P. L.

PY - 1992/10

Y1 - 1992/10

N2 - The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

AB - The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

UR - http://www.scopus.com/inward/record.url?scp=0026938984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026938984&partnerID=8YFLogxK

M3 - Article

C2 - 1416657

VL - 157

SP - 7

EP - 10

JO - The Annals of otology, rhinology & laryngology. Supplement

JF - The Annals of otology, rhinology & laryngology. Supplement

SN - 0096-8056

ER -