Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children

Y. Okamoto, K. Kudo, K. Shirotori, M. Nakazawa, E. Ito, K. Togawa, Janak Patel, P. L. Ogra

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The reverse transcriptase-polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.

Original languageEnglish (US)
Pages (from-to)7-10
Number of pages4
JournalAnnals of Otology, Rhinology and Laryngology
Volume101
Issue number10 II
StatePublished - 1992
Externally publishedYes

Fingerprint

Otitis Media with Effusion
Respiratory Syncytial Viruses
Respiratory Syncytial Virus Infections
Nasopharynx
Otitis Media
Reverse Transcriptase Polymerase Chain Reaction
Disease Outbreaks
Polymerase Chain Reaction

Keywords

  • otitis media with effusion
  • polymerase chain reaction
  • respiratory syncytial virus

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Okamoto, Y., Kudo, K., Shirotori, K., Nakazawa, M., Ito, E., Togawa, K., ... Ogra, P. L. (1992). Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children. Annals of Otology, Rhinology and Laryngology, 101(10 II), 7-10.

Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children. / Okamoto, Y.; Kudo, K.; Shirotori, K.; Nakazawa, M.; Ito, E.; Togawa, K.; Patel, Janak; Ogra, P. L.

In: Annals of Otology, Rhinology and Laryngology, Vol. 101, No. 10 II, 1992, p. 7-10.

Research output: Contribution to journalArticle

Okamoto, Y, Kudo, K, Shirotori, K, Nakazawa, M, Ito, E, Togawa, K, Patel, J & Ogra, PL 1992, 'Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children', Annals of Otology, Rhinology and Laryngology, vol. 101, no. 10 II, pp. 7-10.
Okamoto, Y. ; Kudo, K. ; Shirotori, K. ; Nakazawa, M. ; Ito, E. ; Togawa, K. ; Patel, Janak ; Ogra, P. L. / Detection of genomic sequences of respiratory syncytial virus in otitis media with effusion in children. In: Annals of Otology, Rhinology and Laryngology. 1992 ; Vol. 101, No. 10 II. pp. 7-10.
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