Detection of Human Papillomavirus Type 16 in Plasma Cells

Deborah Payne, Stephen K. Tyring, Mark G. Doherty, Dean Daya, Teh Sheng Chan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chronic benign plasma cell tumor of the cervix, also called chronic plasma cell cervicitis, is a rare disease of unknown etiology, characterized by a heavy infiltration of plasma cells forming granulation tissue. To identify infectious agents associated with this disease, we extracted and analyzed DNA from the 17-year-old paraffin section of the original case report and from granulation tissue surgically removed from a patient at our institution with a chronic benign plasma cell tumor. The DNA from both patients was shown by a polymerase chain reaction (PCR) technique to contain a human papillomavirus 16 (HPV 16) sequence. Genomic Southern analysis of the fresh-frozen tissue confirmed the initial PCR finding. In situ hybridization further demonstrated that the HPV 16 was present in the plasma cells and not a contaminant from the surrounding epithelial tissue. The etiological role of HPV 16, an oncogenic virus associated with cervical carcinoma, in this disease is not yet clear. Our results, however, suggest that the types of cells that are infected by HPV may include cells of lymphoid origin, and that HPV may be associated with chronic benign plasma cell tumors of the cervix.

Original languageEnglish (US)
Pages (from-to)406-412
Number of pages7
JournalGynecologic Oncology
Volume48
Issue number3
DOIs
StatePublished - Mar 1993

Fingerprint

Plasmacytoma
Human papillomavirus 16
Plasma Cells
Granulation Tissue
Cervix Uteri
Uterine Cervicitis
Polymerase Chain Reaction
Oncogenic Viruses
DNA
Rare Diseases
Paraffin
In Situ Hybridization
Epithelium
Lymphocytes
Carcinoma

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

Cite this

Payne, D., Tyring, S. K., Doherty, M. G., Daya, D., & Chan, T. S. (1993). Detection of Human Papillomavirus Type 16 in Plasma Cells. Gynecologic Oncology, 48(3), 406-412. https://doi.org/10.1006/gyno.1993.1071

Detection of Human Papillomavirus Type 16 in Plasma Cells. / Payne, Deborah; Tyring, Stephen K.; Doherty, Mark G.; Daya, Dean; Chan, Teh Sheng.

In: Gynecologic Oncology, Vol. 48, No. 3, 03.1993, p. 406-412.

Research output: Contribution to journalArticle

Payne, D, Tyring, SK, Doherty, MG, Daya, D & Chan, TS 1993, 'Detection of Human Papillomavirus Type 16 in Plasma Cells', Gynecologic Oncology, vol. 48, no. 3, pp. 406-412. https://doi.org/10.1006/gyno.1993.1071
Payne D, Tyring SK, Doherty MG, Daya D, Chan TS. Detection of Human Papillomavirus Type 16 in Plasma Cells. Gynecologic Oncology. 1993 Mar;48(3):406-412. https://doi.org/10.1006/gyno.1993.1071
Payne, Deborah ; Tyring, Stephen K. ; Doherty, Mark G. ; Daya, Dean ; Chan, Teh Sheng. / Detection of Human Papillomavirus Type 16 in Plasma Cells. In: Gynecologic Oncology. 1993 ; Vol. 48, No. 3. pp. 406-412.
@article{43df3d4ad0e0469080da118fb339787b,
title = "Detection of Human Papillomavirus Type 16 in Plasma Cells",
abstract = "Chronic benign plasma cell tumor of the cervix, also called chronic plasma cell cervicitis, is a rare disease of unknown etiology, characterized by a heavy infiltration of plasma cells forming granulation tissue. To identify infectious agents associated with this disease, we extracted and analyzed DNA from the 17-year-old paraffin section of the original case report and from granulation tissue surgically removed from a patient at our institution with a chronic benign plasma cell tumor. The DNA from both patients was shown by a polymerase chain reaction (PCR) technique to contain a human papillomavirus 16 (HPV 16) sequence. Genomic Southern analysis of the fresh-frozen tissue confirmed the initial PCR finding. In situ hybridization further demonstrated that the HPV 16 was present in the plasma cells and not a contaminant from the surrounding epithelial tissue. The etiological role of HPV 16, an oncogenic virus associated with cervical carcinoma, in this disease is not yet clear. Our results, however, suggest that the types of cells that are infected by HPV may include cells of lymphoid origin, and that HPV may be associated with chronic benign plasma cell tumors of the cervix.",
author = "Deborah Payne and Tyring, {Stephen K.} and Doherty, {Mark G.} and Dean Daya and Chan, {Teh Sheng}",
year = "1993",
month = "3",
doi = "10.1006/gyno.1993.1071",
language = "English (US)",
volume = "48",
pages = "406--412",
journal = "Gynecologic Oncology",
issn = "0090-8258",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Detection of Human Papillomavirus Type 16 in Plasma Cells

AU - Payne, Deborah

AU - Tyring, Stephen K.

AU - Doherty, Mark G.

AU - Daya, Dean

AU - Chan, Teh Sheng

PY - 1993/3

Y1 - 1993/3

N2 - Chronic benign plasma cell tumor of the cervix, also called chronic plasma cell cervicitis, is a rare disease of unknown etiology, characterized by a heavy infiltration of plasma cells forming granulation tissue. To identify infectious agents associated with this disease, we extracted and analyzed DNA from the 17-year-old paraffin section of the original case report and from granulation tissue surgically removed from a patient at our institution with a chronic benign plasma cell tumor. The DNA from both patients was shown by a polymerase chain reaction (PCR) technique to contain a human papillomavirus 16 (HPV 16) sequence. Genomic Southern analysis of the fresh-frozen tissue confirmed the initial PCR finding. In situ hybridization further demonstrated that the HPV 16 was present in the plasma cells and not a contaminant from the surrounding epithelial tissue. The etiological role of HPV 16, an oncogenic virus associated with cervical carcinoma, in this disease is not yet clear. Our results, however, suggest that the types of cells that are infected by HPV may include cells of lymphoid origin, and that HPV may be associated with chronic benign plasma cell tumors of the cervix.

AB - Chronic benign plasma cell tumor of the cervix, also called chronic plasma cell cervicitis, is a rare disease of unknown etiology, characterized by a heavy infiltration of plasma cells forming granulation tissue. To identify infectious agents associated with this disease, we extracted and analyzed DNA from the 17-year-old paraffin section of the original case report and from granulation tissue surgically removed from a patient at our institution with a chronic benign plasma cell tumor. The DNA from both patients was shown by a polymerase chain reaction (PCR) technique to contain a human papillomavirus 16 (HPV 16) sequence. Genomic Southern analysis of the fresh-frozen tissue confirmed the initial PCR finding. In situ hybridization further demonstrated that the HPV 16 was present in the plasma cells and not a contaminant from the surrounding epithelial tissue. The etiological role of HPV 16, an oncogenic virus associated with cervical carcinoma, in this disease is not yet clear. Our results, however, suggest that the types of cells that are infected by HPV may include cells of lymphoid origin, and that HPV may be associated with chronic benign plasma cell tumors of the cervix.

UR - http://www.scopus.com/inward/record.url?scp=0027415157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027415157&partnerID=8YFLogxK

U2 - 10.1006/gyno.1993.1071

DO - 10.1006/gyno.1993.1071

M3 - Article

C2 - 8385062

AN - SCOPUS:0027415157

VL - 48

SP - 406

EP - 412

JO - Gynecologic Oncology

JF - Gynecologic Oncology

SN - 0090-8258

IS - 3

ER -