Detection of humoral antigen and antibody by enzyme-linked immunosorbent assay in horses with experimentally induced Ehrlichia equi infection.

R. E. Corstvet, S. D. Gaunt, P. A. Karns, Jere McBride, R. A. Battistini, L. A. Mauterer, F. W. Austin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay (ELISA) was used to detect antigen in plasma and antibody in serum of 3 horses inoculated with Ehrlichia equi. Clinical signs, including rectal temperature, were correlated with the antigen and antibody detection. ELISA was very efficient in detection of serum antibody. Antigen detection using monoclonal antibodies to E. equi and ELISA should be considered as a diagnostic method.

Original languageEnglish (US)
Pages (from-to)37-39
Number of pages3
JournalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Volume5
Issue number1
StatePublished - Jan 1993
Externally publishedYes

Fingerprint

Anaplasma phagocytophilum
Horses
antigen detection
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
antigens
horses
Antigens
antibodies
Antibodies
Infection
infection
antibody detection
Serum
diagnostic techniques
monoclonal antibodies
Monoclonal Antibodies
Temperature
temperature

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

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abstract = "An enzyme-linked immunosorbent assay (ELISA) was used to detect antigen in plasma and antibody in serum of 3 horses inoculated with Ehrlichia equi. Clinical signs, including rectal temperature, were correlated with the antigen and antibody detection. ELISA was very efficient in detection of serum antibody. Antigen detection using monoclonal antibodies to E. equi and ELISA should be considered as a diagnostic method.",
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AU - McBride, Jere

AU - Battistini, R. A.

AU - Mauterer, L. A.

AU - Austin, F. W.

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