Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays

Tang Qing, Masayuki Saijo, Han Lei, Masahiro Niikura, Akihiko Maeda, Tetsuro Ikegami, Wang Xinjung, Ichiro Kurane, Shigeru Morikawa

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.

Original languageEnglish (US)
Pages (from-to)111-116
Number of pages6
JournalJournal of Virological Methods
Volume108
Issue number1
DOIs
StatePublished - Mar 2003
Externally publishedYes

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Keywords

  • Crimean-Congo hemorrhagic fever virus
  • ELISA
  • Immunofluorescence
  • Recombinant nucleoprotein
  • Seroepidemiology

ASJC Scopus subject areas

  • Virology

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