TY - JOUR
T1 - Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays
AU - Qing, Tang
AU - Saijo, Masayuki
AU - Lei, Han
AU - Niikura, Masahiro
AU - Maeda, Akihiko
AU - Ikegami, Tetsuro
AU - Xinjung, Wang
AU - Kurane, Ichiro
AU - Morikawa, Shigeru
N1 - Funding Information:
We would like to thank Dr. B. Shimayi, Bachu County Center for Disease Prevention and Control, for his excellent assistance with this work. We would like to thank Ms X. Zhao and Ms X. Tao, Second Division of Viral Hemorrhagic Fever, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, for their excellent technical assistance with this work. We also thank Ms M. Ogata, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan, for her technical and official assistance. This work was supported by a Grant-in-Aid from the Ministry of Health, Labor and Welfare of Japan, from Japan Food Hygiene Association, Tokyo, Japan.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/3
Y1 - 2003/3
N2 - Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.
AB - Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.
KW - Crimean-Congo hemorrhagic fever virus
KW - ELISA
KW - Immunofluorescence
KW - Recombinant nucleoprotein
KW - Seroepidemiology
UR - http://www.scopus.com/inward/record.url?scp=12244307827&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12244307827&partnerID=8YFLogxK
U2 - 10.1016/S0166-0934(02)00267-7
DO - 10.1016/S0166-0934(02)00267-7
M3 - Article
C2 - 12565161
AN - SCOPUS:12244307827
SN - 0166-0934
VL - 108
SP - 111
EP - 116
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -