Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays

Tang Qing; Masayuki Saijo; Han Lei; Masahiro Niikura; Akihiko Maeda; Tetsuro Ikegami; Wang Xinjung; Ichiro Kurane; Shigeru Morikawa

doi:10.1016/S0166-0934(02)00267-7

Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays

Tang Qing, Masayuki Saijo, Han Lei, Masahiro Niikura, Akihiko Maeda, Tetsuro Ikegami, Wang Xinjung, Ichiro Kurane, Shigeru Morikawa

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.

Original language	English (US)
Pages (from-to)	111-116
Number of pages	6
Journal	Journal of Virological Methods
Volume	108
Issue number	1
DOIs	https://doi.org/10.1016/S0166-0934(02)00267-7
State	Published - Mar 2003
Externally published	Yes

Keywords

Crimean-Congo hemorrhagic fever virus
ELISA
Immunofluorescence
Recombinant nucleoprotein
Seroepidemiology

ASJC Scopus subject areas

Virology

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10.1016/S0166-0934(02)00267-7

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Qing, T., Saijo, M., Lei, H., Niikura, M., Maeda, A., Ikegami, T., Xinjung, W., Kurane, I., & Morikawa, S. (2003). Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays. Journal of Virological Methods, 108(1), 111-116. https://doi.org/10.1016/S0166-0934(02)00267-7

Qing, T, Saijo, M, Lei, H, Niikura, M, Maeda, A, Ikegami, T, Xinjung, W, Kurane, I & Morikawa, S 2003, 'Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays', Journal of Virological Methods, vol. 108, no. 1, pp. 111-116. https://doi.org/10.1016/S0166-0934(02)00267-7

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title = "Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays",

abstract = "Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50\% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.",

keywords = "Crimean-Congo hemorrhagic fever virus, ELISA, Immunofluorescence, Recombinant nucleoprotein, Seroepidemiology",

author = "Tang Qing and Masayuki Saijo and Han Lei and Masahiro Niikura and Akihiko Maeda and Tetsuro Ikegami and Wang Xinjung and Ichiro Kurane and Shigeru Morikawa",

note = "Funding Information: We would like to thank Dr. B. Shimayi, Bachu County Center for Disease Prevention and Control, for his excellent assistance with this work. We would like to thank Ms X. Zhao and Ms X. Tao, Second Division of Viral Hemorrhagic Fever, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, for their excellent technical assistance with this work. We also thank Ms M. Ogata, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan, for her technical and official assistance. This work was supported by a Grant-in-Aid from the Ministry of Health, Labor and Welfare of Japan, from Japan Food Hygiene Association, Tokyo, Japan. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2003",

month = mar,

doi = "10.1016/S0166-0934(02)00267-7",

language = "English (US)",

volume = "108",

pages = "111--116",

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T1 - Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays

AU - Qing, Tang

AU - Saijo, Masayuki

AU - Lei, Han

AU - Niikura, Masahiro

AU - Maeda, Akihiko

AU - Ikegami, Tetsuro

AU - Xinjung, Wang

AU - Kurane, Ichiro

AU - Morikawa, Shigeru

N1 - Funding Information: We would like to thank Dr. B. Shimayi, Bachu County Center for Disease Prevention and Control, for his excellent assistance with this work. We would like to thank Ms X. Zhao and Ms X. Tao, Second Division of Viral Hemorrhagic Fever, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, for their excellent technical assistance with this work. We also thank Ms M. Ogata, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan, for her technical and official assistance. This work was supported by a Grant-in-Aid from the Ministry of Health, Labor and Welfare of Japan, from Japan Food Hygiene Association, Tokyo, Japan. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2003/3

Y1 - 2003/3

N2 - Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.

AB - Crimean-Congo hemorrhagic fever virus is a tick-borne virus that causes severe hemorrhagic symptoms with an up to 50% mortality rate in humans. Wild and domestic animals, such as sheep, cattle and goats, are the reservoirs. The recombinant nucleoprotein-based Crimean-Congo hemorrhagic fever virus antibody detection systems for sheep sera were developed by enzyme-linked immunosorbent assay (ELISA) and an indirect immunofluorescence assay techniques. The samples used for evaluation were 80 sera collected from sheep in a Crimean-Congo hemorrhagic fever-endemic area (western part of the Xinjiang Uygur Autonomous Region) and 39 sera collected from sheep in a disease-free region (Shandong province, eastern China). The ELISA and indirect immunofluorescence assay using recombinant nucleoprotein of the virus proved to have high sensitivity and specificity for detecting the immunoglobulin G antibodies to the virus in sheep sera. Within this limited number of samples, the recombinant nucleoprotein-based ELISA and indirect immunofluorescence assay are considered to be useful tools for seroepidemiological study of virus infections in sheep sera.

KW - Crimean-Congo hemorrhagic fever virus

KW - ELISA

KW - Immunofluorescence

KW - Recombinant nucleoprotein

KW - Seroepidemiology

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ER -

Detection of immunoglobulin G to Crimean-Congo hemorrhagic fever virus in sheep sera by recombinant nucleoprotein-based enzyme-linked immunosorbent and immunofluorescence assays

Abstract

Keywords

ASJC Scopus subject areas

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