Detection of pathogenic Leptospira spp. Infections among mammals captured in the Peruvian Amazon basin region

J. E. Bunnell, C. L. Hice, D. M. Watts, V. Montrueil, R. B. Tesh, J. M. Vinetz

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

To identify potential zoonotic reservoirs of pathogenic leptospires in the Peruvian Amazon basin, wild mammals were trapped from July 1997 to December 1998 near the city of Iquitos. After extraction of nucleic acids from animal kidneys, DNA of pathogenic leptospires was identified by polymerase chain reaction (PCR) assays using one of two primer sets, one amplifying a region of the 23S rRNA gene, and the other amplifying a gene fragment specific for Leptospira spp (G1/G2 primers). Overall, 29% (40 of 136) of the mammals tested showed evidence of renal infection by Leptospira spp., including 20% (13 of 64) of the rodents, 39% (20 of 51) of the marsupials, and 35% (7 of 20) of the chiropterans (bats). Marsupials and chiropterans were implicated as more significant reservoir hosts of leptospires pathogenic to humans than previously recognized.

Original languageEnglish (US)
Pages (from-to)255-258
Number of pages4
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume63
Issue number5-6
StatePublished - 2000

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Leptospira
Marsupialia
Mammals
Kidney
Zoonoses
Infection
rRNA Genes
Nucleic Acids
Rodentia
Polymerase Chain Reaction
DNA
Genes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Bunnell, J. E., Hice, C. L., Watts, D. M., Montrueil, V., Tesh, R. B., & Vinetz, J. M. (2000). Detection of pathogenic Leptospira spp. Infections among mammals captured in the Peruvian Amazon basin region. American Journal of Tropical Medicine and Hygiene, 63(5-6), 255-258.

Detection of pathogenic Leptospira spp. Infections among mammals captured in the Peruvian Amazon basin region. / Bunnell, J. E.; Hice, C. L.; Watts, D. M.; Montrueil, V.; Tesh, R. B.; Vinetz, J. M.

In: American Journal of Tropical Medicine and Hygiene, Vol. 63, No. 5-6, 2000, p. 255-258.

Research output: Contribution to journalArticle

Bunnell, JE, Hice, CL, Watts, DM, Montrueil, V, Tesh, RB & Vinetz, JM 2000, 'Detection of pathogenic Leptospira spp. Infections among mammals captured in the Peruvian Amazon basin region', American Journal of Tropical Medicine and Hygiene, vol. 63, no. 5-6, pp. 255-258.
Bunnell, J. E. ; Hice, C. L. ; Watts, D. M. ; Montrueil, V. ; Tesh, R. B. ; Vinetz, J. M. / Detection of pathogenic Leptospira spp. Infections among mammals captured in the Peruvian Amazon basin region. In: American Journal of Tropical Medicine and Hygiene. 2000 ; Vol. 63, No. 5-6. pp. 255-258.
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