Detection of pathological zinc accumulation in neurons: Methods for autopsy, biopsy, and cultured tissue

Sang Won Suh, Kathy Listiack, Brent Bell, Jefferson Chen, Massoud Motamedi, David Silva, Gorm Danscher, William Whetsell, Richard Thompson, Christopher Frederickson

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

It has been repeatedly shown that synaptically released zinc contributes to excitotoxic neuronal injury in ischemia, epilepsy, and mechanical head trauma. Such zinc-induced injury leaves an unmistakable 'footprint' in the injured neurons, allowing an easy and unambiguous postmortem diagnosis. This footprint is the presence of weakly bound, histochemically reactive zinc in the cytoplasm of the perikaryon and proximal dendrites. Such staining appears to be a necessary and sufficient marker for zinc-induced neuronal injury. Here we show how to prepare and stain tissue from biopsy, autopsy, or experimental animal sources for maximal contrast and visibility of zinc- injured neurons.

Original languageEnglish (US)
Pages (from-to)969-972
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume47
Issue number7
DOIs
StatePublished - Jul 1999

Keywords

  • Dendrites
  • Neuronal injury
  • Perikarya
  • Staining
  • Zinc

ASJC Scopus subject areas

  • Anatomy
  • Histology

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    Suh, S. W., Listiack, K., Bell, B., Chen, J., Motamedi, M., Silva, D., Danscher, G., Whetsell, W., Thompson, R., & Frederickson, C. (1999). Detection of pathological zinc accumulation in neurons: Methods for autopsy, biopsy, and cultured tissue. Journal of Histochemistry and Cytochemistry, 47(7), 969-972. https://doi.org/10.1177/002215549904700715