Detection of severe fever with thrombocytopenia syndrome virus by reverse transcription-cross-priming amplification coupled with vertical flow visualization

Lunbiao Cui, Yiyue Ge, Xian Qi, Gaolian Xu, Haijing Li, Kangchen Zhao, Bin Wu, Zhiyang Shi, Xiling Guo, Lin Hu, Qimin You, Li Hong Zhang, Alexander N. Freiberg, Xuejie Yu, Hua Wang, Minghao Zhou, Yi Wei Tang

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49 Scopus citations

Abstract

A virus known as severe fever with thrombocytopenia syndrome virus (SFTSV) was recently identified as the etiological agent of severe fever with thrombocytopenia syndrome (SFTS) in China. Reliable laboratory detection and identification of this virus are likely to become clinically and epidemiologically desirable. We developed a nearly instrument-free, simple molecular method which incorporates reverse transcription-cross-priming amplification (RT-CPA) coupled with a vertical flow (VF) visualization strip for rapid detection of SFTSV. The RT-CPA-VF assay targets a conserved region of theMsegment of the SFTSV genome and has a limit of detection of 100 copies per reaction, with no cross-reaction with other vector-borne bunyaviruses and bacterial pathogens. The performance of the RT-CPA-VF assay was determined with 175 human plasma specimens collected from 89 clinically suspected SFTS patients and 86 healthy donors. The sensitivity and specificity of the assay were 94.1% and 100.0%, respectively, compared with a combination of virus culture and real-time RT-PCR. The entire procedure, from specimen processing to result reporting, can be completed within 2 h. The simplicity and nearly instrument-free platform of the RT-CPA-VF assay make it practical for point-of-care testing.

Original languageEnglish (US)
Pages (from-to)3881-3885
Number of pages5
JournalJournal of Clinical Microbiology
Volume50
Issue number12
DOIs
StatePublished - Dec 1 2012

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ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Cui, L., Ge, Y., Qi, X., Xu, G., Li, H., Zhao, K., Wu, B., Shi, Z., Guo, X., Hu, L., You, Q., Zhang, L. H., Freiberg, A. N., Yu, X., Wang, H., Zhou, M., & Tang, Y. W. (2012). Detection of severe fever with thrombocytopenia syndrome virus by reverse transcription-cross-priming amplification coupled with vertical flow visualization. Journal of Clinical Microbiology, 50(12), 3881-3885. https://doi.org/10.1128/JCM.01931-12