Abstract
MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.
Original language | English (US) |
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Pages (from-to) | 147-151 |
Number of pages | 5 |
Journal | Vector-Borne and Zoonotic Diseases |
Volume | 9 |
Issue number | 2 |
DOIs | |
State | Published - Apr 1 2009 |
Keywords
- Aedes
- Arbovirus(es)
- Birds
- Culex
- Diagnostics
- Mosquito(es)
- Vector-borne
- Zoonosis
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases
- Virology