Detection of tick-borne pathogens by masstag polymerase chain reaction

Rafal Tokarz, Vishal Kapoor, James E. Samuel, Donald Bouyer, Thomas Briese, W. Ian Lipkin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalVector-Borne and Zoonotic Diseases
Volume9
Issue number2
DOIs
StatePublished - Apr 1 2009

Fingerprint

Ticks
Polymerase Chain Reaction
Multiplex Polymerase Chain Reaction
Communicable Diseases
Mass Spectrometry
Molecular Weight

Keywords

  • Aedes
  • Arbovirus(es)
  • Birds
  • Culex
  • Diagnostics
  • Mosquito(es)
  • Vector-borne
  • Zoonosis

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Virology

Cite this

Detection of tick-borne pathogens by masstag polymerase chain reaction. / Tokarz, Rafal; Kapoor, Vishal; Samuel, James E.; Bouyer, Donald; Briese, Thomas; Lipkin, W. Ian.

In: Vector-Borne and Zoonotic Diseases, Vol. 9, No. 2, 01.04.2009, p. 147-151.

Research output: Contribution to journalArticle

Tokarz, Rafal ; Kapoor, Vishal ; Samuel, James E. ; Bouyer, Donald ; Briese, Thomas ; Lipkin, W. Ian. / Detection of tick-borne pathogens by masstag polymerase chain reaction. In: Vector-Borne and Zoonotic Diseases. 2009 ; Vol. 9, No. 2. pp. 147-151.
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