Detection of tick-borne pathogens by masstag polymerase chain reaction

Rafal Tokarz, Vishal Kapoor, James E. Samuel, Donald H. Bouyer, Thomas Briese, W. Ian Lipkin

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalVector-Borne and Zoonotic Diseases
Volume9
Issue number2
DOIs
StatePublished - Apr 1 2009

Keywords

  • Aedes
  • Arbovirus(es)
  • Birds
  • Culex
  • Diagnostics
  • Mosquito(es)
  • Vector-borne
  • Zoonosis

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases
  • Virology

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