Detection of tick-borne pathogens by masstag polymerase chain reaction

Rafal Tokarz; Vishal Kapoor; James E. Samuel; Donald H. Bouyer; Thomas Briese; W. Ian Lipkin

doi:10.1089/vbz.2008.0088

Detection of tick-borne pathogens by masstag polymerase chain reaction

Rafal Tokarz, Vishal Kapoor, James E. Samuel, Donald H. Bouyer, Thomas Briese, W. Ian Lipkin

Pathology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

Original language	English (US)
Pages (from-to)	147-151
Number of pages	5
Journal	Vector-Borne and Zoonotic Diseases
Volume	9
Issue number	2
DOIs	https://doi.org/10.1089/vbz.2008.0088
State	Published - Apr 1 2009

Keywords

Aedes
Arbovirus(es)
Birds
Culex
Diagnostics
Mosquito(es)
Vector-borne
Zoonosis

ASJC Scopus subject areas

Microbiology
Infectious Diseases
Virology

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10.1089/vbz.2008.0088

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abstract = "MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.",

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AU - Tokarz, Rafal

AU - Kapoor, Vishal

AU - Samuel, James E.

AU - Bouyer, Donald H.

AU - Briese, Thomas

AU - Lipkin, W. Ian

PY - 2009/4/1

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AB - MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and implementation of a MassTag PCR panel for surveillance of microbes implicated in tick-vectored infectious diseases.

KW - Aedes

KW - Arbovirus(es)

KW - Birds

KW - Culex

KW - Diagnostics

KW - Mosquito(es)

KW - Vector-borne

KW - Zoonosis

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Detection of tick-borne pathogens by masstag polymerase chain reaction

Abstract

Keywords

ASJC Scopus subject areas

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